	   SnapGene      &tcgcgcgtttcggtgatgacggtgaaaacctctgacacatgcagctcccggagacggtcacagcttgtctgtaagcggatgccgggagcagacaagcccgtcagggcgcgtcagcgggtgttggcgggtgtcggggctggcttaactatgcggcatcagagcagattgtactgagagtgcaccatatgcggtgtgaaataccgcacagatgcgtaaggagaaaataccgcatcaggcgccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaagggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtgaattgacgcgtattgggatttaaccctagaaagatagtctgcgtaaaattgacgcatgcattcttgaaatattgctctctctttctaaatagcgcgaatccgtcgctgtgcatttaggacatctcagtcgccgcttggagctcccgtgaggcgtgcttgtcaatgcggtaagtgtcactgattttgaactataacgaccgcgtgagtcaaaatgacgcatgattatcttttacgtgacttttaagatttaactcatacgataattatattgttatttcatgttctacttacgtgataacttattatatatatattttcttgttatagatatcggcgcgccggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggtctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgagcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgagccaccATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCGCTAGAGGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACGCCCTGGTTCCTGGAACAATTGCTTTTACAGATGCACATATCGAGGTGAACATCACGTACGCGGAATACTTCGAAATGTCCGTTCGGTTGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAATCGTCGTATGCAGTGAAAACTCTCTTCAATTCTTTATGCCGGTGTTGGGCGCGTTATTTATCGGAGTTGCAGTTGCGCCCGCGAACGACATTTATAATGAACGTGAATTGCTCAACAGTATGAACATTTCGCAGCCTACCGTAGTGTTTGTTTCCAAAAAGGGGTTGCAAAAAATTTTGAACGTGCAAAAAAAATTACCAATAATCCAGAAAATTATTATCATGGATTCTAAAACGGATTACCAGGGATTTCAGTCGATGTACACGTTCGTCACATCTCATCTACCTCCCGGTTTTAATGAATACGATTTTGTACCAGAGTCCTTTGATCGTGACAAAACAATTGCACTGATAATGAACTCCTCTGGATCTACTGGGTTACCTAAGGGTGTGGCCCTTCCGCATAGAACTGCCTGCGTCAGATTCTCGCATGCCAGAGATCCTATTTTTGGCAATCAAATCATTCCGGATACTGCGATTTTAAGTGTTGTTCCATTCCATCACGGTTTTGGAATGTTTACTACACTCGGATATTTGATATGTGGATTTCGAGTCGTCTTAATGTATAGATTTGAAGAAGAGCTGTTTTTACGATCCCTTCAGGATTACAAAATTCAAAGTGCGTTGCTAGTACCAACCCTATTTTCATTCTTCGCCAAAAGCACTCTGATTGACAAATACGATTTATCTAATTTACACGAAATTGCTTCTGGGGGCGCACCTCTTTCGAAAGAAGTCGGGGAAGCGGTTGCAAAACGCTTCCATCTTCCAGGGATACGACAAGGATATGGGCTCACTGAGACTACATCAGCTATTCTGATTACACCCGAGGGGGATGATAAACCGGGCGCGGTCGGTAAAGTTGTTCCATTTTTTGAAGCGAAGGTTGTGGATCTGGATACCGGGAAAACGCTGGGCGTTAATCAGAGAGGCGAATTATGTGTCAGAGGACCTATGATTATGTCCGGTTATGTAAACAATCCGGAAGCGACCAACGCCTTGATTGACAAGGATGGATGGCTACATTCTGGAGACATAGCTTACTGGGACGAAGACGAACACTTCTTCATAGTTGACCGCTTGAAGTCTTTAATTAAATACAAAGGATACCAGGTGGCCCCCGCTGAATTGGAGTCGATATTGTTACAACACCCCAACATCTTCGACGCGGGCGTGGCAGGTCTTCCCGACGATGACGCCGGTGAACTTCCCGCCGCCGTTGTTGTTTTGGAGCACGGAAAGACGATGACGGAAAAAGAGATCGTGGATTACGTCGCCAGTCAAGTAACAACCGCGAAAAAGTTGCGCGGAGGAGTTGTGTTTGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTAAtgactcgagtctagagggcccgtttaaacccgctgatcagcctcgactgtgccttctagttgccagccatctgttgtttgcccctcccccgtgccttccttgaccctggaaggtgccactcccactgtcctttcctaataaaatgaggaaattgcatcgcattgtctgagtaggtgtcattctattctggggggtggggtggggcaggacagcaagggggaggattgggaagacaatagcaggcatgctggggatgcggtgggctctatggttttttggtacccttaagcacgtggtatccgtcgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtcgaggtgagccccacgttctgcttcactctccccatctcccccccctccccacccccaattttgtatttatttattttttaattattttgtgcagcgatgggggcggggggggggggggggcgcgcgccaggcggggcggggcggggcgaggggcggggcggggcgaggcggagaggtgcggcggcagccaatcagagcggcgcgctccgaaagtttccttttatggcgaggcggcggcggcggcggccctataaaaagcgaagcgcgcggcgggcgggagtcgctgcgcgctgccttcgccccgtgccccgctccgccgccgcctcgcgccgcccgccccggctctgactgaccgcgttactcccacaggtgagcgggcgggacggcccttctcctccgggctgtaattagcgcttggtttaatgacggcttgtttcttttctgtggctgcgtgaaagccttgaggggctccgggagggccctttgtgcggggggagcggctcggggggtgcgtgcgtgtgtgtgtgcgtggggagcgccgcgtgcggctccgcgctgcccggcggctgtgagcgctgcgggcgcggcgcggggctttgtgcgctccgcagtgtgcgcgaggggagcgcggccgggggcggtgccccgcggtgcggggggggctgcgaggggaacaaaggctgcgtgcggggtgtgtgcgtgggggggtgagcagggggtgtgggcgcgtcggtcgggctgcaaccccccctgcacccccctccccgagttgctgagcacggcccggcttcgggtgcggggctccgtacggggcgtggcgcggggctcgccgtgccgggcggggggtggcggcaggtgggggtgccgggcggggcggggccgcctcgggccggggagggctcgggggaggggcgcggcggcccccggagcgccggcggctgtcgaggcgcggcgagccgcagccattgccttttatggtaatcgtgcgagagggcgcagggacttcctttgtcccaaatctgtgcggagccgaaatctgggaggcgccgccgcaccccctctagcgggcgcggggcgaagcggtgcggcgccggcaggaaggaaatgggcggggagggccttcgtgcgtcgccgcgccgccgtccccttctccctctccagcctcggggctgtccgcggggggacggctgccttcgggggggacggggcagggcggggttcggcttctggcgtgtgaccggcggctctagagcctctgctaaccatgttcatgccttcttctttttcctacagctcctgggcaacgtgctggttattgtgctgtctcatcattttggcaaagaattcgaagctagcttcgcgatcgcctcgaggccaccGGATCCTAGGCCCGGGtcctcaggtgcaggctgcctatcagaaggtggtggctggtgtggccaatgccctggctcacaaataccactgagatctttttccctctgccaaaaattatggggacatcatgaagccccttgagcatctgacttctggctaataaaggaaatttattttcattgcaatagtgtgttggaattttttgtgtctctcactcggaaggacatatgggagggcaaatcatttaaaacatcagaatgagtatttggtttagagtttggcaacatatgcccatatgctggctgccatgaacaaaggttggctataaagaggtcatcagtatatgaaacagccccctgctgtccattccttattccatagaaaagccttgacttgaggttagattttttttatattttgttttgtgttatttttttctttaacatccctaaaattttccttacatgttttactagccagatttttcctcctctcctgactactcccagtcatagctgtccctcttctcttatggagatcttctaccgggtaggggaggcgcttttcccaaggcagtctggagcatgcgctttagcagccccgctgggcacttggcgctacacaagtggcctctggcctcgcacacattccacatccaccggtaggcgccaaccggctccgttctttggtggccccttcgcgccaccttctactcctcccctagtcaggaagttcccccccgccccgcagctcgcgtcgtgcaggacgtgacaaatggaagtagcacgtctcactagtctcgtgcagatggacagcaccgctgagcaatggaagcgggtaggcctttggggcagcggccaatagcagctttgctccttcgctttctgggctcagaggctgggaaggggtgggtccgggggcgggctcaggggcgggctcaggggcggggcgggcgcccgaaggtcctccggaggcccggcattctgcacgcttcaaaagcgcacgtctgccgcgctgttctcctcttcctcatctccgggcctttcgacctcacgtggccaccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtccccagggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgatccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgacagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtagctagctattctctagcggccgctttgttactttatagaagaaattttgagtttttgtttttttttaataaataaataaacataaataaattgtttgttgaatttattattagtatgtaagtgtaaatataataaaacttaatatctattcaaattaataaataaacctcgatatacagaccgataaaacacatgcgtcaattttacgcatgattatctttaacgtacgtcacaatatgattatctttctagggttaaagcatggtcatagctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttagaaaaactcatcgagcatcaaatgaaactgcaatttattcatatcaggattatcaataccatatttttgaaaaagccgtttctgtaatgaaggagaaaactcaccgaggcagttccataggatggcaagatcctggtatcggtctgcgattccgactcgtccaacatcaatacaacctattaatttcccctcgtcaaaaataaggttatcaagtgagaaatcaccatgagtgacgactgaatccggtgagaatggcaaaagtttatgcatttctttccagacttgttcaacaggccagccattacgctcgtcatcaaaatcactcgcatcaaccaaaccgttattcattcgtgattgcgcctgagcgagacgaaatacgcgatcgctgttaaaaggacaattacaaacaggaatcgaatgcaaccggcgcaggaacactgccagcgcatcaacaatattttcacctgaatcaggatattcttctaatacctggaatgctgttttcccagggatcgcagtggtgagtaaccatgcatcatcaggagtacggataaaatgcttgatggtcggaagaggcataaattccgtcagccagtttagtctgaccatctcatctgtaacatcattggcaacgctacctttgccatgtttcagaaacaactctggcgcatcgggcttcccatacaatcgatagattgtcgcacctgattgcccgacattatcgcgagcccatttatacccatataaatcagcatccatgttggaatttaatcgcggcctagagcaagacgtttcccgttgaatatggctcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtc  S   ~   0   ,   V   5   -   %      @      %      b   '   7   A   1       $   1   0   4               &      +            1   &   '   9      #         $      -   $   &             f   #   +   V   .   &   !      >      (   2   :   4   4   ?   -   %      -   "   %               %      *          #   .   !   $   "   1   T   =   &   $   *   0         &         #   (            (   %      (   0   ?   !      -                   !      (   *                  (            3   *   "   <   '   -      +   +      +      8   &      -                  4          %      5                   )   -   *   !      %   <   !   4   5   j   7   &      3   <   )         /   *   "   &   !   *      -   $   %      '   ,   R       .   B   (            /         +   F   &   8   P   .   "      $       ,   !      !   !   -   )             $   >   !      0               !      4   7   $   (   $   -   7      "   N   #      '   "   )      2   .   <      (   C        ~  =>F|}2Di		/0JN).3:?Du34567AUbcdeop +,12>?@PQRSYc|.U()./+a"&0 S ?P~F3]	Kk}m }B!-AT}/09, "$, s } Yd Gx7B?
			OVfq d)I^b"H&&V g  3:Ej;YS1}	*/4;@E3v 8w%2 3Z_cV|a*A %5 1 T  G`#4m5iN~".B0I1:-R#%- Q<S "f
L?<JaCCsd  !r"$$$/%%% L @6H	T	^		;
x


lwZ^Y j!A"""%#n#$$%[&  &~n!BU~/[F $@   t ~  1>yC@q	eS$.Wgr36=A.Qh|45  !!"#$ Z2H#Bu*J_ $!>!c!y!!!"}#% e8+=V9			`
R.EeBV(=_5"#I&& 7 !PQ
jj c"""#+%b h z  6M!A;>af,<2

&+05<AFKXk!x38JV[`q|3k+2Bx8 W  !$%&'  %
=*
R|[[AdWV!!b#r##5%j%7    eT~b4Pbw9K&M4Fj$9N2F}]27r  !%&A R}4Fkx
Z	I%;ByN]&b>Iawt !$%&1 vP{d$8IQs?#/TO1O2;03 w!`#3%&  2   9H5
nQO_8."S###e$$  WJvC0	
j	3M3!!!"#%%1 U  0ar7y
6gjC^J<R.{ s!#a$%&W&0 ;=A

DM@b0D#tXe  !!!J""O#0%;&4    9RT,Iw9	#@=Kwuj_ ; !9"w""#;#U##j$%r&&& !#Xf	g
##$$$$%&g& E,			D; !!s"$  M  AXi	[_D,po#(% N	<
y

mxP  $%%\&&  P?h#I	U	_		
D



$QZ,6Qk!."""&##W$%% *7		

S	a:!B"M"U"""w#s$$+  4 taA'AG6?"MCVTWI & h"#g$
&& ! Z P	rUp&0B C!"$%& wy"3o\G H!##$ OE[ex/\o !R%1 ?z@DT	
W``u47@w$7]6  !!"p"$&    UuV!Awwu	hhv}###U$$' u   7^r	L
XZst>/5{  9  D$2 pK	
&PfT	%/Q\|'BRi}
p  !& +K`  ~!!"# F  [z3I4lBC8vL%!?!d!"J#%_% $n
Y< z!!!"~#k&&  `l		
(_5fC%$ w Vw9x:	N

S*-}0>CO!%& 	/8s	}`!""#J$z$J&-  f,2^	a
k

0AyFU">t6Z " ""D##$I%~%&$ L]>W5r	Q
Xlfz1W)n|   	!!f#$&&  
"[mtkf	
G`#}Q Lk ,%%  8 c m o*9YEv		
gQ9R@'Gk/ "#,$A% B 
E".!d""#N%P&& 
Oc1	*t8oZ 5!#f J q {   0D8<gv*9= P',1=BLVYi",1S	69KY<a}G[&,T0r)3v}!"%Y&# 7N?bpB
Cu6G=\~Ry9 u!#c$q$%+  k  "SM

qfO^{
rx%:X  &V  i   BXUt-3 ln?y4EW4PlDCe`'Cdr  !!4"[$$$%&. ( W  >r }\lc\!BX)XegW!Z!p!("+"6"c#$$& I&	!	+
V>jtH!$"#s##$k%&!   d	
SLEk2f #$<$$%6%%%>&   W	
58e4jb %&>   3FU
5c:LN5G%:/3l`9^qas   !!"w$$ 'D
cGncg!!p%( >  &2Zrp)]vAQk6Rhu8xM  $%%2 + f/px'Om~3EUf ) h t   !!k"($&: P 5 j$'G`cru~5JbFu A#4   /`y{

c37h#"_fCp6^'K!P!]!!$ $$E%y%&4 4kSk~l[J
L(RNnl-J(zOFx !!]$%? _ cG'	E_,JZ&'<I'e[;? Tx !%%&- f <cwX	\		j9t@n$0^-`-]"$G&&%  _%R
vY ZE i!@""$#Q#m#$$$$% =pdR2APP'3<4   x!#-   L Q|e 	%JJU #2@Mv1 V !a#$4%&&"   8I6	

PPfN/2 r!!!2%&%  :@e8		
C/!!8"I""T###%q&&   


R`+9oO -"L"""v#% 3 Y Oo@B!$f$	&  6U?StK

v!o"##$& 
kA'^
X4K!""%%% UKw1]|/7WkxN !!!""e##H% n	X	De		
FfD!. 4!M%* I p Ts8OBh>DFZ%S/q| t!"b$$X&  V  
7kKW3=diGe Y!o!'"*"#%&=& * = 21C
ugbfpg   !# ^ biIz6^^m_e,dZ:w!\!!@#$D%%.  ;K<^QW		uXcI1@?uY?"""P##$! ~

B?OMN A!!!H"K"u#1%&&$ T5T>\J

Ej{&wuJ- !""##&" H ] HB
NAlE9$vFdf   !&"?#<&1 :SPV[	

A>NzvkH?LEcM !>""">##~$&T   |\Ux					#	$	%	&	'	:	O		

XLMxystN   !!:";"<"="x"""""""<#=#V####2$}$%s&t&&&&=   ]
-^	(	;	1

$EUu
Y! """
###3$k$ %:%%u&}&&&  }KJyP		Y6`O< !"y"""#W#E$$%y&&$ -^ .		h

<FJF@Z"#L##$$!%h&*   $Y0		~>VaL-###l$$$&2&v&~&&0 ("	)	<		
%H:v*"##$;%% _g3	-
2
:Dbx" ""##4$$$%%% ]~LK	Q	ZI@$t"m%& 	7nE <al f!! "#$$$$a%%z&  FLz		z'!!z"""#X#%  -
qP= !9!"#A$F$$%&&# !QF	{\`gGksz-AGqhp##)%&(  _fG		"E=cK9]-m4O"""#M##U&& BY[u/j	i


(2 [|!|%%i& & N    .p
!XG%-N##$$	%"%  	
s?\bn  k#%&&( J1		xJ K #%8%%w&&%  	Oz

yce#+[M""#3#$$%&3&a&   %Z	=
nWQ  .##m$$%%"&]&(  )	&R)03i>X$g%%%8&M&0 *Qi	*	=	@	V	
%[-;ITU;w7Rl!/"""'###$$%5&&&&? @&#k	`		$
E
|



+!"#5##$$%%@&c&m&&!   $BJ	
6
V


hszj##M$Q$$<%  h	Ty;# +!M! ##t$$$%%%%-   `C	.
3
[
Ec,U ""#!#*####/$5$>$$$%%d%%D&&&  l8{			c:b:U !C"V"""V%[% +4	

;
 !!N""x##%  5 BR	^
:HL@#NDW?!_"% rML	
!pBU,  F!u"#h$& us	p	^7"XJAE"&&! -^b([J*h0%'  2"i"&$o$n% ;  Qcb1mC g!!!"$$( [ N\		
	s8VoFS=s7	WJ "#$$$%%{&* $ Yy	fq'va!!#b%%_&&& " Ln	r
qD!!$  <Mb 		 
?
z#q  \YS!`!##$  Go
4H (!!! x'{pxgI!"$ z{	t

k]{""Y##8$%$&(   P.|ry&60=C] !"]#G$$  ~-	Y		
FY*S "0##B$%S%&  		7f dQ !"#$$s%%&& H
\Nlp>  !:!%3 
XO{AE1R	.||lav{bT{8@ * ;"5G		e

]l4X`hH.Brc q"$%" "<U
Dt5<g^ib!!!!""""|#$*%< y  5@d	<	)
a
HQ{ap8AM1Ejx%H`~7  U!!q##i%%&'  VuzB/xri^;Q2!""###`$ %V&-   D+HWv			
i:^~!!"#:#N##$&:&f&  >gW")		
#P+5P!T"V$'%%+    `vx	TFZ25>dLw.n[ g""#Q%&&+  Cv	
%_Y[tu?6\z|   E  y_k		
37  }!I#^%j&+ v \M
P
j

,/=@T0!|s{ "$y$$}%% A  )8Zs0)sP7n
 %O&8     /WLo
A

Ue
0<NR]}	OC$9cuq !!#Z$& '  Hq	 		U (j #"# $$
% E%q
(.kl!!$#%- O 3&

'2DQg"Y&kI'Ho&5S~ $x%%& ,L\   !l#&& 7	
!""%&p& 
.6@V L% t]=Vcaepo n!!" G  S4}]	I
@!"#&4 {J	N	5DKDMbL """""|$%9%%x&&  2			
 }=C9IwK##$%  \Kmy?&!e!!%`%&%   FPo
z!'1 f Wjry$,\N{!"#& I
dIZJ ""%%&5 )%?	#
{

/:2yi=!#4##L$$$4&7&L&b&l&& k
B	+9T !*!L!!" #)###%C&&&  ,r	
o]K~)g+/D!1"^"%$n$% [m	q

eu`p6V!!#$%^&  a&	>
n
yw#&   ,	X				
X%R   !/#$r%%) x 	W:04;;(
d
3		zCDiS?!!""h%%- C*(ty.		~

OSY*14qP1!!!S"9#&%&9&e&* @  x(	O
+.?S{"$%N&! V%		
'T~1Cjk8""Y$w%% U<Um!"K%o&%  R|\	H

<HC>a""""#{$%&< j>	A	&0.9Viqxx8S!K!"#(###K$%%6&B&K&&&! +q
+	W		m


x\Jt}
~. !0"$$$4 B/$-3			'
c
}

2BRjz7!"!"R""8#v%d&5 p{	G
l

GpTB-=""##J%%%A&n&j gAozno-.+,_`ab			%
&
F
b



1Zoef8?@A,""""Y""""6#7#$$,&-&7  hp'^cla	

<xrzV[g9w#Xu[   Z"E##$$$$%&.&&&&   $4Mh(^)6s	

=Mgr{2o   !R$&  r?Xmb	


Y:$*
!"#$N$$%/&3 %CdK	

+i+*W\ m"x(kY  !["F#$$$< iq_g 		7
R
W
_fUyms{ht{:XvIO\ } g###	$$=%%&(&&&) 	  #5)_17v
H~)0Nh<5hp  !#-%% N*g	
> $R!M$ #$% \nuilt		
Uz%ln  ,!##*$S$&/    %in

asIp|3S_ N!:$u$$%%%* d n    gMnc	


d-V%+0  !m""#$%B%%%E&" +ZFY/

hF:(+ F"""#+####$0$?$$0&& s@D	~	4

RZSl ""#-$O$%Y%e% &S&&! 9 p:Faw	\
Q ;AHW  }""[##6$$&*  C 	,j;]nmF}y!!e""G##O%\%% L			,#)TnZ\""$&- "m&9e

:dXc ;VG/!!!W""$$&&$ D}|	
+!'# lv    D"W%Q&% h`2	8
+< nr0z9J ~  !!!##>%& |h	5	S
X
`g)p| 6!h##
$%' d		 
Vuzt|-uo{wPx O""y#)&, - jr,P	
 Jm#i;[Y#] j  #$%&&R R r |  E69Ch2:>IM(-2>C2|2T
*1=OXb(H'* `"%  0 K !S	:
w
>;IMA !%.%Z&. 
  6  10*8	_
Q-A#-@	-U{4i~ #!=!!"##B  $=`w	+WZj$O7:ILZEi{~\=\16sqw!!$%( u/Ocq~
7H>S]27L-Nz v!#_#d$%& 8+"?vt"W:  v"i$$& OMh		C

C#r$% sN@qD!%SVH% A  !G!##%/ #Tt] oJv		VOgP_{s&#	o  T$  meB;&  v4q	
)
rymY  !"C#I$&&+ b l Nblju	

_"8|Y?K&F-!##+$@%&&F   j RC7u)tpm+z5X;QwBd_&(3"p$%%&&   cV	
4biDfas15";$$$$%%&8 &Y.o\o@5Q_m8twDLT`e( s     !!
"j"'$v$o%P   ._jj
K#&b+M!FEJqt}4E
  !O!\$. e   bo[	
i$m]"_&,D U h!##$$$$$%F&" X    N ?d7		

e.*n1 q!!,"7"$% mRsd	e]~W C !!n""d#$G%$ ) < o 1Sh5 ]d+cY2Yfh!X![!)"$$C%%  \ JGtWi%>t8uI,  %"G"t##&, [OZ]		"		0




WGTr>	X !""	###1$D$$|&! ,'/		,
9G;aE?`)K"#,##$$%1& 
Hpk
#8!##$@$$$l%%! % eZtE	
b8M,Fl"3g"#%{%%T&&!  	r[bw"*~m  #2#j#7%%%!&`&-   Aj5
U

g(SThr$"$P$f%%%?&&&&)   z		Z
b9 T "##.$=$$%%U%Z%c%%% q~o	]
 9!>  E!~"&  # :  XKMbx	Rr I & ;	s

jp[fXR!_!!7$$$  {G}65)Mk<Bc""\##$%&>  4Nc!		-k{_Po;L0Ga~z_}
fb  T!a!"{#$%!   =V	]:Z !f""#_$$P%%& D 
$<r6^O6m	ryz    H#x$$]%0  Gn

@
d;MH&n4jbt !!!##$ 6
 -5do[!"& M		|!"$&  (HE"

 1*HhI )!#& 		p
dv$o*U!!!]"q%! ? ''Us:NXyBRph0!!!"$%%  [
w|hw !J!4 nym]*	;wq~Ynd7SQivy<W  Q"X""$u%+&&7 #3gqf(	

*^e*T)Ll!q'9HN !"$%'&$ m0
u
(/$Ho4
R^gm ##)$9$( fEL}	P
V*    E"l"|""Z#X%&R&$  !|~	 9&l|SmFu !!%%&- , g{		Ilq,/y "(nz" i w   7 / Q /	9
,=1{@Hz'[z "!<!!#$ t.}
s16KGV@  !^##%&" a Iak3(	N!d>!B#H$?%&N   Qi6	"%soL*a:^7Psv AcD^rvK	   !	"&# 0alR6	Z		
hd4\#*^T !$$$$F% 	.	

G$`Dq=_" 7!#C$'    iT
Y
a8ag)7q}+  1#i##!$%T%z%%%%%&" zJ}|ioZ(=LQ!^!"&) m
^OKi|	ex a y   z#^$$$%& 5	!

{0u)!"# &&2 lTf\)		
M)SWvg}(omKvPp&  !!P""t%*&&.   leK		8H.%.k{	Gny!R	Ql #< . ` s-H		!MK)?]O@ZUqy? !!;!%&& kQIn`[(<<]$%%%( e(	

G]/'$Jjhm% ` k   #&C dFKL-.(=>fg\\$   ^ _ !!!#&  ;>  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        2                                                                                                                                                                                            
                                                                                                                                Y                                                        	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     
                                                                                          	                                    6                           +                                                                                        H   	      	                                                                         +                                                                         
                %   +      
                  
         `   >            	         	                           
      ,                           
                  ;                  F   N   f         V         A   7   !            &         0   .   1   %   x                     2                                                                                        
                                                            Y                          	                                                                                                                                                                                                                                                   
                              	            6         +                              H 	  	                         +                         
      % +  
      
   ` >    	   	         
  ,         
      ;      F N f   V   A 7 !    &   0 . 1 % x         &      (Z & )i#c. 5st.}J	!
`**K)'@" !i#z#$
C$	%[!Tv5  JO- cb5$    ZgpC#JV1<ENWc:Re>Ou;u,^bk:}+"$p%%D#
x#%#0
%#
%#0# ||s"Y
|q$r^*	&	!FF	e9e^$E
	dDiX&	J""]$- zQ|Mv6WI&#8
<   1e$Q|7gR.{W&wA\%H`s!	
:%%!
! 6WI&>[Yn	.4 6   s-0(/E;#)=c E%cR A%aE}v%|%%&m$m  
L;
l [&M	zk[ ! ,>D3kB ` 1e`2DEiew$;VQ| &kG
(sp0!E* E"r$r!at6}Z	h]*>.:J==]^ ` ^#$$$$.8
+< !!#>h.Gy$l	,sQ` 'Nsi!r$eB&2 e$% aE}6v%%c|R %k	"S&&Y=!~F%&
0% /3"8lp9

3JlOG\!/L?^ tcy$"$$%6}*:= $ Y
L!6	
`A"L"""v#r$$6*: B""Kh  kG
!
(X!)"j!#	% "*# I^l	k
4# "pr&,1u5			8

<jF /!!!F"""#
$S$$%%S&&&'	~ 6!##9,%%Go 16*:^7Y !	"5J41#[sEp* 0!E"$2&/~h# _k	^4%aE6	A""$*

	!M""w#C*X	

!9#$&/&9&e&aZ	h^O#K^|	eT a z#$$"P&&2 ne$(  F\c:LcG%3XXegas!W!Z!("3EFj
Y	I%; a>[&. Q 	1{?'Zy;!  @g#$F;A"[##$[( \cXXegW!Z!(" Fc:LG%3as!Y
a8T%[_,po#(%#ND!j'cA#(   F5<v *$VY\ic:L69KY`cG}%3XX[erubg0ruasv !W!Z!("%. Q aZ		h^O#1{?K'^Z|	eyT a ;!z#$$   #<$$%%%M  Mb 	zS!`!ld\!F%L @Z^n#%fL l" ]:_$}m AT}
OZ 5 Q^
#q@?=!"G$ fL	]: l"_$&Rsn"$;	p[XR!_!$a'& h"k M
{
%!K)]O@qq { D8g9=',1=B1S	<aG&)W#CyfI		

\3!###$$s@lj_"|SVHK%   M

v
dr-0 ~ !e""#-%O%%f	
)S(Kp& !;b0tXO#+ fpxm h  T `5BI8 [o9uEwv			ls	v@}G%!d!!#%_%

!J"0%"e
d G"$&&WC	
3!y\#8 o9uEwv		v@G##fI	
##$$ 5<v *$VYi69KY`}[rub0ruv %;Rs	p[XR!_!n"$$G		9]"#M#r?
Y#N$R	:H%`2 >%  j RC7u)tpm+z5X;QwBd_&(3"p$%%&  Qi6	"%soL*a:^7Psv AcD^rvK	   !	"&J q {   0D8<gv*9= P',1=BLVYi",1S	69KY<a}G[&,T0r)3v}!"%Y& `l		
(_5fC%* = 21C
ugbfpg   ! i   BXUt-3 ln?y4EW4PlDCe`'Cdr  !!4"[$$$%&6
 -5do[!"&*7		

S	a:!B"M"U"""w#s$$R}4Fkx
Z	I%;ByN]&b>Iawt !$%&/ Q /	9
,=1{@Hz'[z "!<!!#$% eZtE	
b8M,Fl"3g"#%{%%T&&* = ^    2e1Rb}4Fkx
ZiIzCT	
~bu6^I^%4Pbmw9K&;M_e,4Fj$9BN2FdZyN]g}&:b>I]aw27wbftp r g    !\!!!!!@#$$D%%%%&&%    IeZtFPoE		


z!'1r [bdfw Wbjry"$*,8IZ\~M,Fl"3Ngm J  {!""""#2#j###%7%{%%%%%%&!&T&`&&&us	p	^7"XJAE"&&'  Hq	 		U (j #"# $$
%/ Q 0al/Rm6	Z			

9
h,=d4^O\#1{@H*zKi'^[|	ezx T a y    "!<!!!z##$$^$$$$$$F%%&/ Q e    4b/Nc!o[				9

-i,=k{_P$1{m@H]oz";L0'Ga~z_[_}
fz&,D U b   "!<!T!a!h!!"##{##$$$$$$$%%F&("	)	<		
%H:v*"##$;%%  leK		8H.%.k{	Gny!R	Ql #vP{d$8IQs?#/TO1O2;03 w!`#3%& R|\	H

<HC>a""""#{$%& * = 2us=1pC	p			
7u^d7fR2APg"X JPd'3<QbfAEp4 g      x!!!""##$$s%%&&&&N*g	
> $R!M$ #$%   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  )<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="21" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1916-1925" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="20" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7812-7828" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7788-7804" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="379-395" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7881-7902" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-10" range="7836-7842" color="#ffffff" type="standard"/><Segment range="7843-7860" color="#ffffff" type="standard"/><Segment name="-35" range="7861-7866" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="β-globin poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5797-5852" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;rabbit&amp;nbsp;β-globin polyadenylation signal (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3568131&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3568131&quot;&gt;Gil and Proudfoot, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9765-9869" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7311-7432" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3621-3845" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="chicken β-actin promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4260-4537" color="#ffffff" type="standard"/></Feature><Feature recentID="10" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3879-4258" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="PGK promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6186-6685" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mouse phosphoglycerate kinase 1 promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8190-8778" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="6702-7301" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"/></Q></Feature><Feature recentID="6" name="KanR" directionality="2" translationMW="30979.26" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="8949-9764" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-Ia&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to kanamycin in bacteria or G418 (Geneticin®) in eukaryotes&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"/></Q></Feature><Feature recentID="5" name="EF-1α intron A" type="intron" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="968-1906" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;intron upstream of the start codon of human EF-1α&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="chimeric intron" type="intron" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4538-5554" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;chimera between introns from chicken β-actin and rabbit β-globin&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="EF-1α promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="737-1915" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;strong constitutive promoter for human elongation factor EF-1α&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="luciferase" directionality="1" translationMW="60745.28" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1922-3574" color="#ffcc00" type="standard" translated="1"/><Q name="EC_number"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="experiment"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;luc&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;firefly luciferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDAHIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAPANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKSKL*"/></Q></Feature><Feature recentID="1" name="CAG" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3882-5607" color="#a6acb3" type="standard"/></Feature><Feature recentID="0" name="rBG pA" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5657-6178" color="#a6acb3" type="standard"/></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  <Notes>
<UUID>0f198c7c-fdab-41a6-b60a-0723ae93b14d</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>VB205</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="5:9:30">2022.9.21</Created>
<LastModified UTC="5:38:19">2026.1.13</LastModified>
<CreatedBy>Triple Threat</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         Unsaved Enzyme Set                                                                                                    HO               E              Y?                                                                                                                                                                <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="BamHI EcoRI EcoRV FspI HindIII KpnI MfeI NdeI NheI NotI SacII SalI ScaI SpeI XhoI"/></CustomEnzymeSets>
