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      !   $             "         $               )         %         !         #      &         #   (   B   !      #      )            #                "                              "   2   "         '               (   +      O      '      0   2   (      *                        /   =   "                     "      )   %         !         )      ?            +   *      (      .   %   -   '        0  n	#$=jGHyX&jh i 0 ) E  ix;OCy		%@k<Id'8)l;'6RyF    ] .

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A)JW]0e
9}g:  z	
&|Xs z! *  >D:	 q"(Tm<(SS[G $ s$4K_/4	

I*4 
?,GT}18!   ?   9&0u7		
 min;  ^ c	d$%e]+ : Q   Tp|b+:W
t{~8	*06)*  "	
Vs m!ZkvHCEfJ_l# 0 7G@F1JZ[/wa *  =eE!,DZm	=t	?	F2y
[ $ z2,/;:UArw,/9,6$-3&Yf + \(

b0>2EMOQSYLO  0t]%	U
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Q X^bXG;7@CFSFN' G 	K


LK1.4:O~Pm 	{M.Lp>U_h;  QM		
B*8f&"1j Yo7 3S
qD}9 	6
Z|%@Y4n p	{			B5hx%  0


'9QYdx_tU{Q * +   7:,fE		O`gy#Nz s|-w\  *
ibn=.U[, H  d;	rx)UTTP. W -?Rt+FC<<s&)MCo( =  ;}s258		
8!HU{
J+"  *[D
g)OJm5m 0l
bU$!  t%5L`v	
Nr 'xYI_^v2 

-Hva#f9d b
W
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@J-TUF~q0W  IHLey  2p!	
=nG '18	
\Ao=K   v{	 

-j{5<I:><  @ u   ]:-	B	.3V"i  |/_xn
e_&ea ~ #o?K#v0 _  d	%dfT
 q =z'	`&3^0 . O   Ecqv}	7),	

=*[|_   15	XGS2u
+17*S ; |  [{Be=t"8  $ R &(UW'r&HcC|ik'=W_	;r4BcMOeg9Q;  & L y #P`Jy$?5!2D;Q&:b9IL 0 M W=W]{tnQw	n";JlCLwzFg`>   (NCZh 
k6A[<K  d  MhE	
#8!6iyD^m,/ 1 8HA	

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u	Rh/53sv\ ! >.mtST@
QoH`<' 
 )[b7`o>	
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Le3@iontz7* ( RmCNBx	#;Fc&7x(:-%.4'HE g & s=-P
W.V/BS-0r9WZ  3	H   {Y0<r	sZ7j1* ] T

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zK0NWFo=OT^?BEM # R/


CPc?w3m^P   
*	)
Bxra% TZB # u)Z7	
GTNMI	lT&  ~&V
\

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-[,<z4;9U!h  ^&	n^_JS29 N { %Db 6{&AV^
d<3~AFRZbL1d!P- O_L<BY"\z7 %x@aK+=K - >j&	
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V b6Y8GGV/  S



YOw_pn<NS]ADA   }%
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M

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|N}VrP&y3^}$ Ae7?T4		
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]&M9a2p	OI) ON	Q			c

+"3g#Q!=Zfb  & JH	
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C
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!~EzsQ'4~[ P	&
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6+	G_ ;

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mW,&jZg,	  !	H
L208CAg38dp*t( d"	BCT NoajiD $ @ & L\q	|							
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:V@	>M E
(/RT]XPYLV|  1^	
_ZyWyCuZ 

Ee<A{r`eR   "	hbkq{6}PC]  8r
^aoez8:$3 J o   ;-F	+	'!t. , J  Kpgg;		
PNlFO
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#hcu>"#[ go{    '  f 
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Z

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LP--aKi:XnlR6E 	o		5
8
$A(  QeE	N~hw;r{,vLn   :r1	7z~ 
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@
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 ym
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	Jq/M`l>"  n(Y6	Z~^ _k
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nb)[t -Mz
  & B  h  gIX-Ch'b%!   8"		
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x

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)\OW}" 6 	#2C0oz5ukzDU[ le		
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i
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-	TikgcQx9     	F(\r"  r#8^	b	
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Qg3%On.dtGhn6ru ' QBq"-E.%R'i0D ' 	Q.
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A
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^u
v<KNha|H  )O%
 *?Z lV+]Z[  g [i!
e

17nc)s D
SzB\OY=Ld  n  !oq*	
nd7j e vGP*,P"nX  VdpNS		$p97]*/C"~U /  0Nxi%F
k"qz~$E>_b}.-=  7 [ B`I$9DrJ	

;RolDj)Vuw2htd" d3		4

K	9v#,{`*KQD(: 9
v?cI]Ey 2  I
Xf1p{~6LOu>\la  Lm@\R.  H

9_  V\	f			4rx81Vn  fKa(sA
$"   lqP?	
6_z+\-j}E 
[(	t
#f|(4`)  3io|	0	k		|
&hYVW % k   
ysx>

\A!R a +R`	a1'0,VKWwc   LbnU	#


wt[N! 5  "@B=	4twhjGI  ]fOyf1!k C    g>k]SEj (dw) U +Zb.*9	6> ",5 s Gz!vj
HRG dp?    /3VYk$Ffy'AUkwHc 
i$)0s}4
m:5qt]  A-!T
AERDu>hI0  ?p|LE	ZgIaGZG U>ymb(r+  /nupU)	m6p)}B#=a|!T *  Z  _Hc8	3

u>QbCH(t Pv1g'9`  ?U[[			'Qm@(    	phw{	s
{


5"g~3 *Q\aN/svZ.   2j#=x8T\jvb
GQF|!+4o% @ooTK
f|A'q{/F-  Y   PTZv		Z		2
r
z
M!Y}lOu08'     ?in		E{ jk&pE  4  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                     6                                                                                                                                                          
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 $                           *                                                                                  
         *                                            	          
                                                                                                           
               %         
   
               	         F   ,   	                     	                  
                        	                  
            
   !                  (   ?   0      -   8      %   *            Z      !   e   x   ,      $   &   i                                                                                                                                                                          6                                                                          
                                                                                                                                                                                                                                                             $         *                              
   *                	    
                                       
     %   
 
     	   F , 	       	      
        	      
    
 !      ( ? 0  - 8  % *    Z  ! e x ,  $ & i   ?      	  )    	xFU   g#kZ	 }(tpqW g}.$	F?72]e
@
Aryt$	EA((PWXAenENo
 Jet"eHne0AC@`"^k	iW gW gW ge
k}.__
%	+	xytx TAvtb )K'9)^G '(f0x'
  Y Y @B ')s	Y<:, s-_# Q b4_Cc 
)5<d+CsZ J		+ 2 
`QD!Q :b,fO s-[ n   =
!D*
!c  ? 5
rk< u=D
7Q b
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repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4655-4671" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
  <?xml version="1.0"?><Primers nextValidID="1"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/><Primer recentID="0" name="RSV-prompter-F" sequence="atggattggacgaaccactga" description="&lt;html&gt;&lt;body&gt;&lt;/body&gt;&lt;/html&gt;" dateAdded="2024-09-14T08:38:51Z"><BindingSite location="4891-4911" boundStrand="0" annealedBases="atggattggacgaaccactga" meltingTemperature="58"><Component hybridizedRange="4891-4911" bases="atggattggacgaaccactga"/></BindingSite><BindingSite simplified="1" location="4891-4911" boundStrand="0" annealedBases="atggattggacgaaccactga" meltingTemperature="58"><Component hybridizedRange="4891-4911" bases="atggattggacgaaccactga"/></BindingSite></Primer></Primers>
  <Notes>
<UUID>07dedd97-de0a-4af2-ab7b-f17917fa730c</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>VB180</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2021.11.19</Created>
<LastModified UTC="8:38:51">2024.9.14</LastModified>
<CreatedBy>LPreston</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  Y      K         Unsaved Enzyme Set                                                                                                    HO                E              Y?                                                                                                                                                                <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="AgeI BamHI EcoRI FspI HindIII HpaI MfeI NotI SacII SalI ScaI SpeI StuI XhoI XmnI"/></CustomEnzymeSets>
