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q-F|  d;AP a;KX!R  V	[

qJZ` UUl
y
2X0M#1_0  j  'U6	V

Ae*rbcN/ G i|?2	#*EZbh"@!7EJV^f sc'
2&`~;$ w 3e	AGsOMC%:Pq oF m: WO8! f$j1eH		


),q!{S
Zf*   uIs	/
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, BW]S{c  TT:5		Z
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 #Sa	.
>
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oN.R/RYeoG   R^_`}~h()*?<
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u<
-PQ Qn.  / C  N+!
v3	Eaeos=!V! +?:i@&	
=iK`Pt6   vXwF&>R) 0 D Ih^bfp H81FIm*   w}8t,
	

Ft*Up)W M <OW "*		
u4"|?  @;A9	a*Q=& ,'	y			^
 )n>`|jRUg"/&7k    Fj

Lc3z  a
<\4Gu[ wqA6kL	P	/{Tnx)Y%I~&   H7	 
5
"bGy*] #S>D ZYs	"	':/ $  `|5Zem?v  E 0Nc	E>!_9a)   u
_cgmqy2;G_$% 1  !#4S[>?jqii'ckJ  ]bJ-Iq[0*n  3  xB		%38uLP	6'   ~-0F		K
3k&FGX; \    %AU	P5ly8M&G+u j*" ( K9	u:&FX	Z6Vqlc{x 6X	D

v#>Z  @C=P!3#K^~	
xkl5A,3V  e  kpS		OYEbc^}[ N $+		
tW_z"I#i@ AcBN*
8
<l    _(<p	v		2

/besi~<>  8L`N
#/+    x:		

8?TRpJ gSo018' d=\L(	F		_
*lsL}nS/ 'l c i|V	a		%?a kh#. -^bz		QVsng/"  /sj	e.uM><  S_y8u{b  
G
|	
9S)}  kd
;@Xd4w"  /8@	b


KKH'm,Av nI6	]pHA Dt^g2vx  Y	P=]5H\  XtBll	
Lg:*&C&@. t z r7UvM	\		$
H

0PrZw'=Ot	 oRu|P7:JWt+ x,!Q		0|Uyx\qw=MZ#   u	HKc+^$ .>e#T% -  8Gf
		

' :z~FNPT'   Io	@		#U	s9!<(E"    ;6D		
6
cN-6I!Rl{?  W t			|

5; KY[#	I0{!(  ,k=@Z

~
(5Sw{ED    		UJAqgh   ^z$1o
u % Y Xa5`j8$ 5  	|4b_n>@6;Q# 2j}EVCh6[cgfuVwT _   1nOU?N  U"`:^b F d	Fa}#   90	(zM`+  B >%	
;,`dhnr<.d"  v	])7H{>    ]

32)%Z# 6@_{rj		!	a(dlX\9}=    "/\M=^x`/ 2   $@T/kY7L25jkKbzEOw  5?2]	sj "ka!H2YbZ   ^M
>oI c[K]a{U		KmPrf- 	.^i	:8 XHs

J\1Gl+w s yW+T	#
K9O[v&Bs$  ,  5	n?	C		&,J} rM Q;d$   +JX?

{

H4RvzD' 4 W]y#D	n
4abU
7 ^ mT
+     8	:+\'6-(Y,    .z.1	 	W<14wyGa_%  G]h	L

7l=FkH-  VIxWSm"

NZ3IQyq: ]    y~7\9S *;[05v,^h    &d}6R!
'(2HOPUVWXYZ,-./p	
+2mnop+ >  'B
eQzD)%ntk3qo+ :)&c{	[ Wo	4p  PqR	7rCd,4 ) ? (
v;Y	~	
?)47ER&u2m3yar- CLf'BGO	
*x}Gl|8F 4")'f8\wef+j 7;W|	
dS 2Y\d	E
HKCg}?[5d	) S l *]	Q

!$Xps
j6,5q1{ h S_	
[mB8|tDP   DrX
*ZDeWy  QA"4n$	
Wyl6C(-4  Q>QgLq\s-`   TZ		84s~9y @ <q.
 'id, * <  )ow				

*5FPUv3Bn_bi\ 8 : f OGIml ,	@PZF1Scz*| O 
"(	,	
B#m g%CHP		uR,H9G M 	
ReX+.3k~	jAe  z D.Kw'		
	=L`{y}JT$~ 9%F1Xmok  55BdC*g.	+
9
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 J,`!  1&(zOA	
fBg/" -#*D]x= .# 8}k0@0Mf=CLX" K ):q	w	f


>f;T  `<=X3 	3
Xt]  B4			
:ecj?  }    09F

LDGoj$,e  ]hMW\Ly
 3$a	
~$0@6 Q M ZeO
I{B  I  +~d
q
,@@-w|~% !  |I^		
9#Bn95Gq T p  $	H}Mhq%6.5 m `ky;		

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"%Y\dtUk7)Sr2hxK  ~nOeKY`			
;K%\bu&1I2 `/T

pnC#}FE i &cTU
@JWQ% nh{
@l9DY:7I9 eiSHdG	Y


++q\zN)  >]|M ' 	M2~	J_Xsm  us)	tTo0El( E8			`
m_T[fb!y #  Gp5!z4Smx)$.C  RZb	
&bjDlpZ/ ' [ %EW	7%i5 d B#joJR}			Xw@ K_M)
7.rh; Rhk.0  R	cr{	tRW(t=;a .?m	]o&u- s[	k	f/{pvN?Vs'  Ft			95
8O=z ' V j 	TI@z Cg'  i0U{3g^fti=5t:PS  T` A =/
(=c"  ?5		X`6!j]`j8v|< r`zT	9Oe,& + r  *">		J`a+GuTC:c~ g	
Vx'j- =   HcxC:ms4o*l]( Op}				

(6Qw|B`E \1e
Gc~de5+40Rz g  Pmp
[)ZY'{x_ 7 9 ; FHln+-	~0OTA8x{ y 	-Jv&		
AQd-2<QjS}& 04y)	-	


CEWIn 9A	
j9eL:B P | #

znLVK# o  H		

c
G|Lg$m(-4w mNJ	XT
$?"?XEIVH  h{&G7			
pSI^kM FoDIQ	 vRiwYB l-q:t! r	Sf]4h<	7yBUf  >	i\__+% Nb!=	_'l{w9
&k [0Eu*
Y,/Nb}R3wz^y { x		
8
fKUJ  EM	Q>h>oj(  qZ/)Ma|[^z^6v~Q%] L(	I  1*  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                     #                                                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                     	               	                                                                                                                     	                                                                                                                               &                                        	                                             !                                                                            	                                          
         8                                             
   
                     
   
                                                    #   *         0            &      y   r         _   s   *         +   ^                                                                                                                                                                          #                         
                                                                                                                                                                                                                                                                       	     	                                           	                                               &                	               !                            	              
   8               
 
       
 
                  # *   0    &  y r   _ s *   + ^   }  ~  
  :      >b 	"|i*
io^kE(J)f
a
vu t
(^*
t3		Wz^k"$Nix&ixhN%kxKvNl-!kxNkNNS@io)/u t
hI}
	Yb:	S]q	w(,	
zg		:uug$-Dyw7		E	w
S^/SK7
v &
N%~~hxv m
N;tr		
" 3r +j	e>>i]z i"Zbf+M	
xEAu[	c~)x( hqrmMoJ
ERH& /(	,	

Ht5/~
}u
-D>_ 1Dg?MoRv m&

hN%Y[I(hoRnOVb"  /}d6/3\`ao;We/o
R" 3
MC]x\gWb9 *,

v N3=[		=P7$ &bj4	r4\[$			

+<* (,	Z		
K	*3C(by	3%r*	
i~i
)~cx~0 I7@efY[	IQ	:MCx*\
V^yh,  q	&}CMdrmNJX$HUN	)v^	,mzhz	2eskC9OR6H;	BxEWn
S>\	mz	kC96Hhz2esORIQ	vK	O	.H<

yn m,Gzh
Fz	2{esk-Cl9OR"36HrmNJxXE$WnHUYuj	e.uM>T
?IVJ	N	#g Z{t=m	+KNg /tZ{=cJWQ rt=;  w	

SA	
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vnL%DEʯXa7T93	5^6wKtU6aQ8_a!xקqAA9|v b^ϡ_aOg!q5]r旃ZY0eJ4z;Ã[<!2mMF	OɲJnw-ydtlxiMGԩ홞hjUjTꡖor+-EQAF̖㎍]U_|**<Q9B^3F}Y<޵|;[H8e,{QJ|C4p ?N1qgLS=οT0t&@]L@N ςnK=2ƪ!@UMs+݊ok||;<1-!10Q33(<kicNBǁSHL5JNCw;&(LuwA]9iSZ_@]T1*=2ӠX-\QaȩR,IS5n*      6  '<AdditionalSequenceProperties>
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<CustomMapLabel>PBC18.</CustomMapLabel>
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<Created>2024.5.26</Created>
<LastModified>2025.2.7</LastModified>
<AccessionNumber>VB240527-1149kew</AccessionNumber>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  ?               EFKhsckYY3#jA۴΁;赌%"y2a͆rFeh!dfau)hWuFi'U$TZJ*iXm^IfPq]Zm&ER(Ϫ?8 \ݮ/+4< ?1ξ;KB+0r\r=5L1Y03<D@000 ?"S,;(|[|~CRuU48!5N+|J"@=SR}?!~p:݅4zRP/A3}Pq^uR_6@5Iםt\`EmBFv4ӳ}4]p#?}7TL݂J|m`q8@@.v0 AV?|I3r@s2ǟwݱ0-8^B ,0s,S 0̓ġ0Ã]>3A?2
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        '<AdditionalSequenceProperties>
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<DownstreamStickiness>0</DownstreamStickiness>
<UpstreamModification>FivePrimePhosphorylated</UpstreamModification>
<DownstreamModification>FivePrimePhosphorylated</DownstreamModification>
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catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="attB1" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1108-1131" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;recombination site for the Gateway® BP reaction&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;mutant version of&amp;nbsp;&lt;i&gt;att&lt;/i&gt;B&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;BP Clonase™&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="attB2" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="2518-2542" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;recombination site for the Gateway® BP reaction&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;mutant version of&amp;nbsp;&lt;i&gt;att&lt;/i&gt;B&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;BP Clonase™&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="6285-6290" color="#ffffff" type="standard"/><Segment range="6291-6308" color="#ffffff" type="standard"/><Segment name="-10" range="6309-6315" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="T2A" directionality="1" translationMW="1830.01" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1914" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="2517" maxFusedRunOn="2517" detectionMode="exactProteinMatch"><Segment range="1864-1917" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="β-globin poly(A) signal" type="polyA_site" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2685-2740" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;rabbit&amp;nbsp;β-globin polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4237-4341" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="314-554" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RNA polymerase III promoter for human U6 snRNA&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="label"><V text="U6 Promoter"/></Q></Feature><Feature recentID="12" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3756-4211" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="hPGK promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="606-1106" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human phosphoglycerate kinase 1 promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1918-2517" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="2517" maxFusedRunOn="2517"><Segment range="1138-1140" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="1141-1143" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="1144-1854" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="pUC ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5373-5961" color="#ef6cdf" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="/application_notes=Facilitates plasmid replication in E.&#10;coli; regulates high-copy plasmid number (500-700)."/><V text="/description=pUC origin of replication"/><V text="/full_name=pUC origin of replication"/><V text="/official_designation=pUC ori"/><V text="/uuid=b978ad4ff785460db448caf63b728237"/></Q><Q name="label"><V text="pUC ori"/></Q></Feature><Feature recentID="7" name="AmpiciIIin" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4342-5202" color="#5566f5" type="standard"/><Q name="note"><V text="/application_notes=Allows E. coli to be resistant to&#10;ampiciIIin."/><V text="/description=AmpiciIIin resistance gene"/><V text="/full_name=Ampicillin resistance gene"/><V text="/official_designation=Ampicillin"/><V text="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"/></Q><Q name="label"><V text="AmpiciIIin"/></Q></Feature><Feature recentID="6" name="3' ITR" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3276-3510" color="#f58600" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 3' inverted terminal repeat"/><V text="/official_designation=3' ITR"/><V text="/uuid=22382df36c4b4754b7821e5d7876f273"/></Q><Q name="label"><V text="3' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="5" name="rBG pA" directionality="1" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2545-3066" color="#0c5d90" type="standard"/><Q name="note"><V text="/application_notes=Allows transcription termination and&#10;polyadenylation of mRNA transcribed by Pol II RNA polymerase."/><V text="/description=Rabbit beta-globin polyadenylation signal"/><V text="/full_name=Rabbit beta-globin polyadenylation signal"/><V text="/official_designation=rBG pA"/><V text="/uuid=8fa32026eca4441f81c85dc922b69b4a"/></Q><Q name="label"><V text="rBG pA"/></Q></Feature><Feature recentID="4" name="EGFP:T2A:Puro" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-2517" color="#800c0c" type="standard"/><Q name="note"><V text="/application_notes=Allows cells to be visualized by green&#10;fluorescence and resistant to puromycin."/><V text="/description=EGFP and Puro linked by T2A"/><V text="/full_name=EGFP and puromycin dual reporter gene"/><V text="/official_designation=EGFP:T2A:Puro"/><V text="/uuid=1dbf848afb6e407f8db14159237549b9"/></Q><Q name="label"><V text="EGFP:T2A:Puro"/></Q></Feature><Feature recentID="3" name="hPGK promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-1107" color="#db6901" type="standard"/><Q name="note"><V text="/application_notes=Medium-strength promoter."/><V text="/description=Human phosphoglycerate kinase 1 promoter"/><V text="/official_designation=hPGK promoter"/><V text="/uuid=a105ee8f48814e769c9a41c38cfff41e"/></Q><Q name="label"><V text="hPGK promoter"/></Q></Feature><Feature recentID="2" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="314-562" color="#512bbd" type="standard"/><Q name="note"><V text="/application_notes=Pol III promoter; drives expression of&#10;small RNAs."/><V text="/description=Human U6 small nuclear 1 promoter"/><V text="/full_name=Human U6 promoter"/><V text="/official_designation=U6"/><V text="/uuid=0f9979e4676b4788b9441e4543b07317"/></Q><Q name="label"><V text="U6 promoter"/></Q></Feature><Feature recentID="1" name="5' ITR" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-313" color="#946a00" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 5' inverted terminal repeat"/><V text="/official_designation=5' ITR"/><V text="/uuid=cf6f8504beb5412ca461e47810e15037"/></Q><Q name="label"><V text="5' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6546" color="#ffffff" type="standard"/></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  U<Notes>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PBC18.</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created>2024.5.26</Created>
<LastModified>2024.6.26</LastModified>
<AccessionNumber>VB240527-1149kew</AccessionNumber>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                 EFKhsckYY3#jA۴΁;赌%"y2a͆rFeh!dfau)hWuFi'U$TZJ*iXm^IfPq]𪅬f^dU(P
Î2
0.rLn+³!O7.I3X4 ;M3M0C 	"+ͮ5>Lܰÿŷ5*'USC}P1 81\=ԭ2/@cߜ58+n7N?#?#<}SMǮ/=r7ۮ5Q+1lNPA] NyOu˙M\6!zo=lAL;<}E8~K5Gqm/<6D Rc roĝO#>1/'7<+<y=|=Ḛ;"p0<20<J
8<5сC< ,2}@3# C3#G3í<r#A7S:'TB8"XYnZ`m?MzjjdZ"Aـ@ն]XI*D5َُ=
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UXNUj\Ū]VisZzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
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>b2_&>!8g4q3LKHLALLg5|XӃqЫT5ib:cɊ"]jWNasVyPU8'z
k32̷t= Wkr*TT[        '<AdditionalSequenceProperties>
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<DownstreamStickiness>0</DownstreamStickiness>
<UpstreamModification>FivePrimePhosphorylated</UpstreamModification>
<DownstreamModification>FivePrimePhosphorylated</DownstreamModification>
</AdditionalSequenceProperties>

  (<?xml version="1.0"?><Features recycledIDs="7" nextValidID="11"><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6548" color="#ffffff" type="standard"/></Feature><Feature recentID="9" name="5' ITR" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-313" color="#946a00" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 5' inverted terminal repeat"/><V text="/official_designation=5' ITR"/><V text="/uuid=cf6f8504beb5412ca461e47810e15037"/></Q><Q name="label"><V text="5' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="8" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="314-562" color="#512bbd" type="standard"/><Q name="note"><V text="/application_notes=Pol III promoter; drives expression of&#10;small RNAs."/><V text="/description=Human U6 small nuclear 1 promoter"/><V text="/full_name=Human U6 promoter"/><V text="/official_designation=U6"/><V text="/uuid=0f9979e4676b4788b9441e4543b07317"/></Q><Q name="label"><V text="U6 promoter"/></Q></Feature><Feature recentID="6" name="Terminator" directionality="1" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="565-569" color="#1f36a9" type="standard"/><Q name="note"><V text="/application_notes=Allows transcription termination of&#10;small RNA transcribed by Pol III RNA polymerase."/><V text="/description=Pol III transcription terminator"/><V text="/official_designation=Terminator"/><V text="/uuid=95176bf3ff804117b53a563bbd846e19"/></Q><Q name="label"><V text="Terminator"/></Q></Feature><Feature recentID="5" name="hPGK promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="605-1109" color="#db6901" type="standard"/><Q name="note"><V text="/application_notes=Medium-strength promoter."/><V text="/description=Human phosphoglycerate kinase 1 promoter"/><V text="/official_designation=hPGK promoter"/><V text="/uuid=a105ee8f48814e769c9a41c38cfff41e"/></Q><Q name="label"><V text="hPGK promoter"/></Q></Feature><Feature recentID="4" name="EGFP:T2A:Puro" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1140-2519" color="#800c0c" type="standard"/><Q name="note"><V text="/application_notes=Allows cells to be visualized by green&#10;fluorescence and resistant to puromycin."/><V text="/description=EGFP and Puro linked by T2A"/><V text="/full_name=EGFP and puromycin dual reporter gene"/><V text="/official_designation=EGFP:T2A:Puro"/><V text="/uuid=1dbf848afb6e407f8db14159237549b9"/></Q><Q name="label"><V text="EGFP:T2A:Puro"/></Q></Feature><Feature recentID="3" name="rBG pA" directionality="1" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2547-3068" color="#0c5d90" type="standard"/><Q name="note"><V text="/application_notes=Allows transcription termination and&#10;polyadenylation of mRNA transcribed by Pol II RNA polymerase."/><V text="/description=Rabbit beta-globin polyadenylation signal"/><V text="/full_name=Rabbit beta-globin polyadenylation signal"/><V text="/official_designation=rBG pA"/><V text="/uuid=8fa32026eca4441f81c85dc922b69b4a"/></Q><Q name="label"><V text="rBG pA"/></Q></Feature><Feature recentID="2" name="3' ITR" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3278-3512" color="#f58600" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; 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  U<Notes>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PBC18.</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created>2024.5.26</Created>
<LastModified>2024.5.27</LastModified>
<AccessionNumber>VB240527-1149kew</AccessionNumber>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                   ĥEFKhsckYY3#jA۴΁;赌%"y2a͆rFeh!dfau)hWuFi'U$TZJ*iXm^IfPq]B;1~f^dU(P
Î2
0.rLn+³!O7.I3X4 ;M3M0C 	"+ͮ5>Lܰÿŷ5*'USC}P1 81\=ԭ2/@cߜ58+n7N?#?#<}SMǮ/=r7ۮ5Q+1lNPA] NyOu˙M\6!zo=lAL;<}E8~K5Gqm/<6D Rc roĝO#>1/'7<+<y=|=Ḛ;"p0<20<J
8<5сC< ,2}@3# C3#G3í<r#A7S:'TB8"XYnZ`m?MzjjdZ"Aـ@ն]XI*D5َُ=
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>b2_&>!8g4q3LKHLALLg5|XӃqЫT5ib:cɊ"]jWNasVyPU8'z
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   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  U<Notes>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PBC18.</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created>2024.5.26</Created>
<LastModified>2024.5.27</LastModified>
<AccessionNumber>VB240527-1149kew</AccessionNumber>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  A     h  7        7EFKhsckYY3#jA۴΁;赌%"y2a͆rFeh!dfau)hWuFi'U$TZJ*iXm^IfPq]Zm&ER(Ϫ?8 \ݮ/+4< ?1ξ;KB+0r\r=5L1Y03<D@000 ?"S,;(|[|~CRuU48s]=o8`_85eaDg?֎эٱ8IE9qumlcHUZc(jl(\vX\`6"U@(V\US[oUZiW@j'rݻn~*q#/dQ֍nx.>T"xF6=jն"
X{j,XN*ikT=﮳MYƦV 0B&vӚW,%J+ڪ
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Fj]jѪ{N/o~4N)iqeJ*唙j*昙
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s4|z=33=V
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?r/rHWuꆦPƬ>h)Ua8j]UsuYΝjBb3YuecePQ&k]ȳk0ڣ;Es%Qm(H
vnL%DEʯXa7T93	5^6wKtU6aQ8_a!xקqAA9|v b^ϡ_aOg!q5]r旃ZY0eJ4z;Ã[<!2mMF	OɲJnw-ydtlxiMGԩ홞hjUjTꡖor+-EQAF̖㎍]U_|**<Q9B^3F}Y<޵|;[H8e,{QJ|C4p ?N1qgLS=οT0t&@]L@N ςnK=2ƪ!@UMs+݊ok||;<1-!10Q33(<kicNBǁSHL5JNCw;&(LuwA]9iSZ_@]T1*=2ӠX-\QaȩR,IS5n*      6  '<AdditionalSequenceProperties>
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<DownstreamStickiness>0</DownstreamStickiness>
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<DownstreamModification>FivePrimePhosphorylated</DownstreamModification>
</AdditionalSequenceProperties>

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drives expression of&#10;small RNAs."/><V text="/description=Human U6 small nuclear 1 promoter"/><V text="/full_name=Human U6 promoter"/><V text="/official_designation=U6"/><V text="/uuid=0f9979e4676b4788b9441e4543b07317"/></Q><Q name="label"><V text="U6 promoter"/></Q></Feature><Feature recentID="23" name="hPGK promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-1107" color="#db6901" type="standard"/><Q name="note"><V text="/application_notes=Medium-strength promoter."/><V text="/description=Human phosphoglycerate kinase 1 promoter"/><V text="/official_designation=hPGK promoter"/><V text="/uuid=a105ee8f48814e769c9a41c38cfff41e"/></Q><Q name="label"><V text="hPGK promoter"/></Q></Feature><Feature recentID="22" name="LUC:T2A:Puro" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-3450" color="#800c0c" type="standard"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="/application_notes=Allows cells to be visualized by green&#10;fluorescence and resistant to puromycin."/><V text="/description=EGFP and Puro linked by T2A"/><V text="/full_name=EGFP and puromycin dual reporter gene"/><V text="/official_designation=EGFP:T2A:Puro"/><V text="/uuid=1dbf848afb6e407f8db14159237549b9"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="label"><V text="EGFP:T2A:Puro"/></Q></Feature><Feature recentID="21" name="rBG pA" directionality="1" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3478-3999" color="#0c5d90" type="standard"/><Q name="note"><V text="/application_notes=Allows transcription termination and&#10;polyadenylation of mRNA transcribed by Pol II RNA polymerase."/><V text="/description=Rabbit beta-globin polyadenylation signal"/><V text="/full_name=Rabbit beta-globin polyadenylation signal"/><V text="/official_designation=rBG pA"/><V text="/uuid=8fa32026eca4441f81c85dc922b69b4a"/></Q><Q name="label"><V text="rBG pA"/></Q></Feature><Feature recentID="20" name="3' ITR" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4209-4443" color="#f58600" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; 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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PBC18.</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created>2024.5.26</Created>
<LastModified>2025.2.7</LastModified>
<AccessionNumber>VB240527-1149kew</AccessionNumber>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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        '<AdditionalSequenceProperties>
<UpstreamStickiness>0</UpstreamStickiness>
<DownstreamStickiness>0</DownstreamStickiness>
<UpstreamModification>FivePrimePhosphorylated</UpstreamModification>
<DownstreamModification>FivePrimePhosphorylated</DownstreamModification>
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arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="hPGK promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="606-1106" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human phosphoglycerate kinase 1 promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="PuroR" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="1918-2517" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="9" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="2517" maxFusedRunOn="2517"><Segment range="1138-1140" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="1141-1143" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="1144-1854" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="8" name="pUC ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5373-5961" color="#ef6cdf" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="/application_notes=Facilitates plasmid replication in E.&#10;coli; regulates high-copy plasmid number (500-700)."/><V text="/description=pUC origin of replication"/><V text="/full_name=pUC origin of replication"/><V text="/official_designation=pUC ori"/><V text="/uuid=b978ad4ff785460db448caf63b728237"/></Q><Q name="label"><V text="pUC ori"/></Q></Feature><Feature recentID="7" name="AmpiciIIin" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4342-5202" color="#5566f5" type="standard"/><Q name="note"><V text="/application_notes=Allows E. coli to be resistant to&#10;ampiciIIin."/><V text="/description=AmpiciIIin resistance gene"/><V text="/full_name=Ampicillin resistance gene"/><V text="/official_designation=Ampicillin"/><V text="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"/></Q><Q name="label"><V text="AmpiciIIin"/></Q></Feature><Feature recentID="6" name="3' ITR" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3276-3510" color="#f58600" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 3' inverted terminal repeat"/><V text="/official_designation=3' ITR"/><V text="/uuid=22382df36c4b4754b7821e5d7876f273"/></Q><Q name="label"><V text="3' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="5" name="rBG pA" directionality="1" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2545-3066" color="#0c5d90" type="standard"/><Q name="note"><V text="/application_notes=Allows transcription termination and&#10;polyadenylation of mRNA transcribed by Pol II RNA polymerase."/><V text="/description=Rabbit beta-globin polyadenylation signal"/><V text="/full_name=Rabbit beta-globin polyadenylation signal"/><V text="/official_designation=rBG pA"/><V text="/uuid=8fa32026eca4441f81c85dc922b69b4a"/></Q><Q name="label"><V text="rBG pA"/></Q></Feature><Feature recentID="4" name="EGFP:T2A:Puro" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1138-2517" color="#800c0c" type="standard"/><Q name="note"><V text="/application_notes=Allows cells to be visualized by green&#10;fluorescence and resistant to puromycin."/><V text="/description=EGFP and Puro linked by T2A"/><V text="/full_name=EGFP and puromycin dual reporter gene"/><V text="/official_designation=EGFP:T2A:Puro"/><V text="/uuid=1dbf848afb6e407f8db14159237549b9"/></Q><Q name="label"><V text="EGFP:T2A:Puro"/></Q></Feature><Feature recentID="3" name="hPGK promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-1107" color="#db6901" type="standard"/><Q name="note"><V text="/application_notes=Medium-strength promoter."/><V text="/description=Human phosphoglycerate kinase 1 promoter"/><V text="/official_designation=hPGK promoter"/><V text="/uuid=a105ee8f48814e769c9a41c38cfff41e"/></Q><Q name="label"><V text="hPGK promoter"/></Q></Feature><Feature recentID="2" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="314-562" color="#512bbd" type="standard"/><Q name="note"><V text="/application_notes=Pol III promoter; drives expression of&#10;small RNAs."/><V text="/description=Human U6 small nuclear 1 promoter"/><V text="/full_name=Human U6 promoter"/><V text="/official_designation=U6"/><V text="/uuid=0f9979e4676b4788b9441e4543b07317"/></Q><Q name="label"><V text="U6 promoter"/></Q></Feature><Feature recentID="1" name="5' ITR" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-313" color="#946a00" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 5' inverted terminal repeat"/><V text="/official_designation=5' ITR"/><V text="/uuid=cf6f8504beb5412ca461e47810e15037"/></Q><Q name="label"><V text="5' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6546" color="#ffffff" type="standard"/></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  U<Notes>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PBC18.</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created>2024.5.26</Created>
<LastModified>2024.6.26</LastModified>
<AccessionNumber>VB240527-1149kew</AccessionNumber>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                 EFKhsckYY3#jA۴΁;赌%"y2a͆rFeh!dfau)hWuFi'U$TZJ*iXm^IfPq]𪅬f^dU(P
Î2
0.rLn+³!O7.I3X4 ;M3M0C 	"+ͮ5>Lܰÿŷ5*'USC}P1 81\=ԭ2/@cߜ58+n7N?#?#<}SMǮ/=r7ۮ5Q+1lNPA] NyOu˙M\6!zo=lAL;<}E8~K5Gqm/<6D Rc roĝO#>1/'7<+<y=|=Ḛ;"p0<20<J
8<5сC< ,2}@3# C3#G3í<r#A7S:'TB8"XYnZ`m?MzjjdZ"Aـ@ն]XI*D5َُ=
eD-tkOBf"oZbj-?SoJZ\Dfb=Rxye&Z&B姦ux'UTDXT)dQZ*YYY]bR%fYWեWv2֩͆rrfn@eZkO,k?!,uʧ\W2ץvNLQt4X1`L3iL 29ޏLL:rë;jHp߱U^!$ªKܮ+]wn溡
UXNUj\Ū]VisZzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|XӃqЫT5ib:cɊ"]jWNasVyPU8'z
k32̷t= Wkr*TT[        '<AdditionalSequenceProperties>
<UpstreamStickiness>0</UpstreamStickiness>
<DownstreamStickiness>0</DownstreamStickiness>
<UpstreamModification>FivePrimePhosphorylated</UpstreamModification>
<DownstreamModification>FivePrimePhosphorylated</DownstreamModification>
</AdditionalSequenceProperties>

  (<?xml version="1.0"?><Features recycledIDs="7" nextValidID="11"><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6548" color="#ffffff" type="standard"/></Feature><Feature recentID="9" name="5' ITR" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-313" color="#946a00" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 5' inverted terminal repeat"/><V text="/official_designation=5' ITR"/><V text="/uuid=cf6f8504beb5412ca461e47810e15037"/></Q><Q name="label"><V text="5' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="8" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="314-562" color="#512bbd" type="standard"/><Q name="note"><V text="/application_notes=Pol III promoter; drives expression of&#10;small RNAs."/><V text="/description=Human U6 small nuclear 1 promoter"/><V text="/full_name=Human U6 promoter"/><V text="/official_designation=U6"/><V text="/uuid=0f9979e4676b4788b9441e4543b07317"/></Q><Q name="label"><V text="U6 promoter"/></Q></Feature><Feature recentID="6" name="Terminator" directionality="1" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="565-569" color="#1f36a9" type="standard"/><Q name="note"><V text="/application_notes=Allows transcription termination of&#10;small RNA transcribed by Pol III RNA polymerase."/><V text="/description=Pol III transcription terminator"/><V text="/official_designation=Terminator"/><V text="/uuid=95176bf3ff804117b53a563bbd846e19"/></Q><Q name="label"><V text="Terminator"/></Q></Feature><Feature recentID="5" name="hPGK promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="605-1109" color="#db6901" type="standard"/><Q name="note"><V text="/application_notes=Medium-strength promoter."/><V text="/description=Human phosphoglycerate kinase 1 promoter"/><V text="/official_designation=hPGK promoter"/><V text="/uuid=a105ee8f48814e769c9a41c38cfff41e"/></Q><Q name="label"><V text="hPGK promoter"/></Q></Feature><Feature recentID="4" name="EGFP:T2A:Puro" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1140-2519" color="#800c0c" type="standard"/><Q name="note"><V text="/application_notes=Allows cells to be visualized by green&#10;fluorescence and resistant to puromycin."/><V text="/description=EGFP and Puro linked by T2A"/><V text="/full_name=EGFP and puromycin dual reporter gene"/><V text="/official_designation=EGFP:T2A:Puro"/><V text="/uuid=1dbf848afb6e407f8db14159237549b9"/></Q><Q name="label"><V text="EGFP:T2A:Puro"/></Q></Feature><Feature recentID="3" name="rBG pA" directionality="1" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2547-3068" color="#0c5d90" type="standard"/><Q name="note"><V text="/application_notes=Allows transcription termination and&#10;polyadenylation of mRNA transcribed by Pol II RNA polymerase."/><V text="/description=Rabbit beta-globin polyadenylation signal"/><V text="/full_name=Rabbit beta-globin polyadenylation signal"/><V text="/official_designation=rBG pA"/><V text="/uuid=8fa32026eca4441f81c85dc922b69b4a"/></Q><Q name="label"><V text="rBG pA"/></Q></Feature><Feature recentID="2" name="3' ITR" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3278-3512" color="#f58600" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 3' inverted terminal repeat"/><V text="/official_designation=3' ITR"/><V text="/uuid=22382df36c4b4754b7821e5d7876f273"/></Q><Q name="label"><V text="3' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="1" name="AmpiciIIin" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4344-5204" color="#5566f5" type="standard"/><Q name="note"><V text="/application_notes=Allows E. coli to be resistant to&#10;ampiciIIin."/><V text="/description=AmpiciIIin resistance gene"/><V text="/full_name=Ampicillin resistance gene"/><V text="/official_designation=Ampicillin"/><V text="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"/></Q><Q name="label"><V text="AmpiciIIin"/></Q></Feature><Feature recentID="0" name="pUC ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5375-5963" color="#ef6cdf" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="/application_notes=Facilitates plasmid replication in E.&#10;coli; regulates high-copy plasmid number (500-700)."/><V text="/description=pUC origin of replication"/><V text="/full_name=pUC origin of replication"/><V text="/official_designation=pUC ori"/><V text="/uuid=b978ad4ff785460db448caf63b728237"/></Q><Q name="label"><V text="pUC ori"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  U<Notes>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>PBC18.</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created>2024.5.26</Created>
<LastModified>2024.5.27</LastModified>
<AccessionNumber>VB240527-1149kew</AccessionNumber>
<TransformedInto>unspecified</TransformedInto>
</Notes>
                   ĥEFKhsckYY3#jA۴΁;赌%"y2a͆rFeh!dfau)hWuFi'U$TZJ*iXm^IfPq]B;1~f^dU(P
Î2
0.rLn+³!O7.I3X4 ;M3M0C 	"+ͮ5>Lܰÿŷ5*'USC}P1 81\=ԭ2/@cߜ58+n7N?#?#<}SMǮ/=r7ۮ5Q+1lNPA] NyOu˙M\6!zo=lAL;<}E8~K5Gqm/<6D Rc roĝO#>1/'7<+<y=|=Ḛ;"p0<20<J
8<5сC< ,2}@3# C3#G3í<r#A7S:'TB8"XYnZ`m?MzjjdZ"Aـ@ն]XI*D5َُ=
eD-tkOBf"oZbj-?SoJZ\Dfb=Rxye&Z&B姦ux'UTDXT)dQZ*YYY]bR%fYWեWv2֩͆rrfn@eZkO,k?!,uʧ\W2ץvNLQt4X1`L3iL 29ޏLL:rë;jHp߱U^!$ªKܮ+]wn溡
UXNUj\Ū]VisZzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|XӃqЫT5ib:cɊ"]jWNasVyPU8'z
k32̷t= Wkr*TT[        '<AdditionalSequenceProperties>
<UpstreamStickiness>0</UpstreamStickiness>
<DownstreamStickiness>0</DownstreamStickiness>
<UpstreamModification>FivePrimePhosphorylated</UpstreamModification>
<DownstreamModification>FivePrimePhosphorylated</DownstreamModification>
</AdditionalSequenceProperties>

  <?xml version="1.0"?><Features nextValidID="11"><Feature recentID="10" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-6596" color="#ffffff" type="standard"/></Feature><Feature recentID="9" name="5' ITR" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-313" color="#946a00" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 5' inverted terminal repeat"/><V text="/official_designation=5' ITR"/><V text="/uuid=cf6f8504beb5412ca461e47810e15037"/></Q><Q name="label"><V text="5' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="8" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="314-562" color="#512bbd" type="standard"/><Q name="note"><V text="/application_notes=Pol III promoter; drives expression of&#10;small RNAs."/><V text="/description=Human U6 small nuclear 1 promoter"/><V text="/full_name=Human U6 promoter"/><V text="/official_designation=U6"/><V text="/uuid=0f9979e4676b4788b9441e4543b07317"/></Q><Q name="label"><V text="U6 promoter"/></Q></Feature><Feature recentID="7" name="Scramble[shRNA#1]" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="565-612" color="#d84e4e" type="standard"/><Q name="note"><V text="/application_notes=Target: None in human and mouse."/><V text="/official_designation=Scramble[shRNA#1]"/><V text="/uuid=0366278213fc44a69ab2a7b94dbb32db"/></Q><Q name="label"><V text="Scramble[shRNA#1]"/><V text="nonstandard type:  shRNA"/></Q></Feature><Feature recentID="6" name="Terminator" directionality="1" type="terminator" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="613-617" color="#1f36a9" type="standard"/><Q name="note"><V text="/application_notes=Allows transcription termination of&#10;small RNA transcribed by Pol III RNA polymerase."/><V text="/description=Pol III transcription terminator"/><V text="/official_designation=Terminator"/><V text="/uuid=95176bf3ff804117b53a563bbd846e19"/></Q><Q name="label"><V text="Terminator"/></Q></Feature><Feature recentID="5" name="hPGK promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="653-1157" color="#db6901" type="standard"/><Q name="note"><V text="/application_notes=Medium-strength promoter."/><V text="/description=Human phosphoglycerate kinase 1 promoter"/><V text="/official_designation=hPGK promoter"/><V text="/uuid=a105ee8f48814e769c9a41c38cfff41e"/></Q><Q name="label"><V text="hPGK promoter"/></Q></Feature><Feature recentID="4" name="EGFP:T2A:Puro" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1188-2567" color="#800c0c" type="standard"/><Q name="note"><V text="/application_notes=Allows cells to be visualized by green&#10;fluorescence and resistant to puromycin."/><V text="/description=EGFP and Puro linked by T2A"/><V text="/full_name=EGFP and puromycin dual reporter gene"/><V text="/official_designation=EGFP:T2A:Puro"/><V text="/uuid=1dbf848afb6e407f8db14159237549b9"/></Q><Q name="label"><V text="EGFP:T2A:Puro"/></Q></Feature><Feature recentID="3" name="rBG pA" directionality="1" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2595-3116" color="#0c5d90" type="standard"/><Q name="note"><V text="/application_notes=Allows transcription termination and&#10;polyadenylation of mRNA transcribed by Pol II RNA polymerase."/><V text="/description=Rabbit beta-globin polyadenylation signal"/><V text="/full_name=Rabbit beta-globin polyadenylation signal"/><V text="/official_designation=rBG pA"/><V text="/uuid=8fa32026eca4441f81c85dc922b69b4a"/></Q><Q name="label"><V text="rBG pA"/></Q></Feature><Feature recentID="2" name="3' ITR" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3326-3560" color="#f58600" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 3' inverted terminal repeat"/><V text="/official_designation=3' ITR"/><V text="/uuid=22382df36c4b4754b7821e5d7876f273"/></Q><Q name="label"><V text="3' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="1" name="AmpiciIIin" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4392-5252" color="#5566f5" type="standard"/><Q name="note"><V text="/application_notes=Allows E. coli to be resistant to&#10;ampiciIIin."/><V text="/description=AmpiciIIin resistance gene"/><V text="/full_name=Ampicillin resistance gene"/><V text="/official_designation=Ampicillin"/><V text="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"/></Q><Q name="label"><V text="AmpiciIIin"/></Q></Feature><Feature recentID="0" name="pUC ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5423-6011" color="#ef6cdf" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="/application_notes=Facilitates plasmid replication in E.&#10;coli; regulates high-copy plasmid number (500-700)."/><V text="/description=pUC origin of replication"/><V text="/full_name=pUC origin of replication"/><V text="/official_designation=pUC ori"/><V text="/uuid=b978ad4ff785460db448caf63b728237"/></Q><Q name="label"><V text="pUC ori"/></Q></Feature></Features>
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<CustomMapLabel>PBC18.</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created>2024.5.26</Created>
<LastModified>2024.5.27</LastModified>
<AccessionNumber>VB240527-1149kew</AccessionNumber>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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8TGd=qtz;["vKox_iBz)a+f?@ C0OlU~ ӘKswIN˳7JR-{Jғ[bfR׃0@4\+}8"{$n%^1쁙cܱ86;HAm+skozEo"`u7i_//<cPgNbIRm2i6:<3΄ <v킲b?EWN *8PpH1rs&#@^L<3b_m|HzSc%@!dB+xd}'N*ISx?Ffj0#sF2y|::HА ğM9'vcP)QF4.DK5Xì=ͩΒ-"C*60yQnpu&Q%?@oSB@j
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"o믍P@8%kbi+Jd?0,qWiR(isJĸHL3-ĺI+w/5(]fl6!=dpj ~&^ZGJ,楺W}Cy !Mȶ"VlVyq;tdC|)QU?YO|ނlj|n6/q5vNy8ޞ]E&#/PF98䧵usd쾎   	97  ^΁g    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EGRGSLLTCGDVEENPGP"/></Q></Feature><Feature recentID="15" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="7413-7418" color="#ffffff" type="standard"/><Segment range="7419-7436" color="#ffffff" type="standard"/><Segment name="-10" range="7437-7443" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="attB2" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="3646-3670" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;BP Clonase™&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;mutant version of&amp;nbsp;&lt;i&gt;att&lt;/i&gt;B&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;recombination site for the Gateway® BP reaction&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="attB1" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1108-1131" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;BP Clonase™&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;mutant version of&amp;nbsp;&lt;i&gt;att&lt;/i&gt;B&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;recombination site for the Gateway® BP reaction&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7377-7398" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="attB4" directionality="1" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="582-602" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;BP Clonase™&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;mutant version of &lt;i&gt;att&lt;/i&gt;B&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core recombination site for the Gateway® BP reaction&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4701-4719" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="21" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7512-7530" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4726-4742" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7451-7467" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; 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  <Notes>
<UUID>0223d475-36af-4aa1-be89-3515db84c629</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>VB272</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2024.5.25</Created>
<LastModified UTC="2:2:48">2025.2.7</LastModified>
<AccessionNumber>VB240527-1149kew</AccessionNumber>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y      K         Unsaved Enzyme Set                                                                                                    HO  d              E              Y?                                                                                                                                                               -<?xml version="1.0"?><EnzymeVisibilities vals="EcoRI:0,FalI:0,PvuI:0,AhdI:0,BamHI:0,EcoRV:0,DraIII:0,AscI:0,PasI:0,ApaLI:0,MfeI:0,PflMI:0,BplI:0,PflFI:0,PaqCI:0,PstI:0,SpeI:0,EagI:0,BstXI:0,BsmBI:0,AgeI:0,BlpI:0,PaeR7I:0,Eco53kI:0,XcmI:0,PspOMI:0,BsmI:0,ApaI:0,AloI:0,BglI:0,BstBI:0,BsgI:0,AlwNI:0,BclI:0,BstAPI:0,CspCI:0,BsiWI:0,Acc65I:0,HindIII:0,XhoI:0,ArsI:0,AjuI:0,PciI:0,ScaI:0,AclI:0,KpnI:0,NaeI:0,PpuMI:0,Tth111I:0,Bsu36I:0,NcoI:0,NgoMIV:0,HincII:0,PacI:0,BsaI:0,PspXI:0,SgrAI:0,Esp3I:0,BsoBI:0,AvaI:0,PfoI:0,RsrII:0,BglII:0,XmnI:0,NsiI:0,SacI:0"/>
   <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="BamHI EcoRI EcoRV FspI HindIII KpnI MfeI NdeI NheI NotI SacII SalI ScaI SpeI XhoI"/></CustomEnzymeSets>
