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 7kq d "CPh9	*2` <}NR;( 8 S /y)PSZJvTdg(I(  
6b8


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lj ?c% R *5OaY


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#C%"{gJ O P klmS|"#$4*ght{FY"#$\] !wxyzLMZdef78n2) ne+mu|GZy 	+!G}^[}93  }5Xi	>y<@!No nS60[z	HtU6\~!  1'2bTp;GL~Opm:4%  Zo,anv}h	85(' fzH,"xY_	= -  Lm			\Ca[ % ;  ~oMjc 
y=O! l6Yf	x

<_d"Z  2	,~?z>vA_0p (  8f`1{	T~u+ 1 o7:zAIV(e \	"  > ] TC76Dx]|& Ko3:Ujq<H?kPq;   2%Qc(4E	)J:r%n5  #&	M   (T5  c     :!'qi	h{)g#  L[ -bo,Rl%"-  #;t~66?^ p0wN9/e(hFM z `	@	H&Z`hsk  C{I#OW
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Ox' MV"+ t	D
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 	7

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"Gl
t1elv)JS&wS" ?		UmL`3,3t 2  ?:
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=qAc\+`] ]	
8T#CYz2 =	{
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o

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b;
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?n#`EP^Gz F1x$
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6[" 
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s
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j?x   RNo			
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M:bq`zP`im# 	d	

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.
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U~|1
lWnQ>P-    zqO6		{uQFinit#  H++,/	H		26.@J$ Rg

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WflszEXxB|[;?KYc  0m/g	w5 $ :  1LKe	
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G

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z

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r


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JYFC
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u	4


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z2ksLL #mL	i&1'IJK@ABy  1  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                      1                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        	   	                                         
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                                                      	                                                                                	                                   	         "                                           
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                     	      	                     	      
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         	                        "   <   '      (   )      $   $            P         `   k   %          !   [                                                                                                                                                                           1                                                                                                                                                                                                                                                                                    	 	                 
          
                    	                            	             	   "               
                                     	    	                  6 # 
   
     
       	  	       	  
 
   	        " < '  ( )  $ $    P   ` k %    ! [   z  {  |  |D    V  cp` *	+	u			4
d

MP+$_uC
ajt 	~
OQJN@jou	4
;[:		)
A
g

H9koxb<KQ#w,h:AJhF
5Ls	us	8	us	u					CrxPi 	
	k_0_8

uqI w!j8p*dVY?VUX@&	q	m
,	
^F@_;	[	Um\nu|
$*,d	VY@'  	i7i7M		R%R'	_i7		
E,	@[ 	`hk
,	@ Mv@	&	q		m

,	c
^BF@=mgx 	i d
|[AM		
Jjk0)LUR#	^ fJ

)Cs	-.	x 		N~irR)a	R'	
_i7&[AM
0)wu[ 7
+


Lk8,I1aq	J)< [ z r		Mv~c?	*"C(	
i	
#E	
$RfE	5a

?			i7a/j	s

g9l0&Dg	  sZ
	
|KQFBWKAV
K'=gm<`	A]X lv		~s	4Fxj	|kr/I 9eS
Qft5kMSe~S
nKhMqJs
gx0	TEajHN6hQftke~5MSSB
97e}	
p  Qf
			/
>
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ot=5kQM)Se z_~S|
kgr/Ix0A
Uq):C%		,`Q5I C
f:x

O 9
z^k OW)J	giV *x+G		LJm-0h[ x9


z^Ok +,/	@ "Cl(qtycDi|
+,
d
\M'~-Q	3Ufqcy	Q

MXpu`c,UXb|  yyP]m)jt"
3^l -	{	e0Tc!		
C&6N
+ aja&Mxb0{N
uF#xQ
pc	
ua&M{NyMX  
			/
>
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o=Q) z_ "+C,/	l(@IV1dy< b				(
U
^Oq"lTce+pWY j				-
0
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<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created>2024.10.12</Created>
<LastModified>2024.10.12</LastModified>
<CreatedBy>L Preston</CreatedBy>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  +b      M           s"K5`JMv>AU7IB	itp.q}hhjR>NvW}>T:Ԓ"?.({^Do UH3tS_DLQ@0ǂsZED`#2iL ElUk
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M4iUPnQp;H{r-iTꕥVVI.)ߛ欭"F^qn5|sýMe}=<4R^oKZ(II+=)dr=;,&
qJvbTE%(<)
`xkx=cI}KkY"c|J;Ye3ݓEU#\U(Fb_׆M}2Ut5XUPP1{gY6)Y]Y 3vT~E}a_Uɷ3*p_wcN:.%0cMO@̴mF7ٌ"7F2PEYm5P1iNT b81(	s*Kq%\pS  '<AdditionalSequenceProperties>
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   <Notes>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created>2024.10.12</Created>
<LastModified>2024.10.12</LastModified>
<CreatedBy>L Preston</CreatedBy>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  ,     M           s"K5`JMv>AU7IB	itp.q}hhjR>NvW}>T:Ԓ"?.({^Do UH3tS_DLQ@0ǂsZED`#2iL ElUk
P TUY]&S@NEƳJ[Wm8՞ӝ}EigI5戍ATwGQUIG7ML=AZRRIU}MQH#DEUHNM4`3L8pGZh&HMZ9M^. SN58QNUx>c%tjq֝g{hQl}5EZhԨh*]V&eZkeI:	
Y*HY*PYQBDGDtokH\l)zQT  	4QddGgWUէUVI?I~v&9!*IX
 j.X[\bu?ՀC"pBe4NʒFρ8FjV1AH7lJ Z>DP0	^R' |$\p*<]Ӆ:nCos+>>^2ːS,G;û>㣵r*ʮ+Fu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|߂6O>[_uZThx&^b9:>mh`ڮ[࣋A(C\ "5
 =Ӿίh>>8 87Z;>"~/9[<;>̺Ϭİn<'QPӉEP xZqFQ꣢xnYSHRH!	yKHVM$-i&^QXYDAz*hӦX)Օ5mիueMx
m9??o{Ҽ>RHAk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>^l%Ǧx4	tM9+H yÝQtn3OJ~UDWW8$
M4iUPnQp;H{r-iTꕥVVI.)ߛ欭"F^qn5|sýMe}=<4R^oKZ(II+=)dr=;,&
qJvbTE%(<)
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operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4870-4891" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4777-4793" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4801-4817" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7377-7393" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Factor Xa site" directionality="2" translationMW="473.54" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="3951" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="3877" maxFusedRunOn="3877" detectionMode="exactProteinMatch"><Segment range="3952-3963" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
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   <Notes>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created>2024.10.12</Created>
<LastModified>2024.10.12</LastModified>
<CreatedBy>L Preston</CreatedBy>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  +b      M           s"K5`JMv>AU7IB	itp.q}hhjR>NvW}>T:Ԓ"?.({^Do UH3tS_DLQ@0ǂsZED`#2iL ElUk
P TUY]&S@NEƳJ[Wm8՞ӝ}EigI5戍ATwGQUIG7ML=AZRRIU}MQH#DEUHNM4`3L8pGZh&HMZ9M^. SN58QNUx>c%tjq֝g{hQl}5EZhԨh*]V&eZkeI:	
Y*HY*PYQBDGDtokH\l)zQT  	4QddGgWUէUVI?I~v&9!*IX
 j.X[\bu?ՀC"pBe4NʒFρ8FjV1AH7lJ Z>DP0	^R' |$\p*<]Ӆ:nCos+>>^2ːS,G;û>㣵r*ʮ+Fu?p=<̬3|lL|N5s2vP0q<.D"l?3`(L+4F|?PÔ|˳54,<L ϯ_L;|߂6O>[_uZThx&^b9:>mh`ڮ[࣋A(C\ "5
 =Ӿίh>>8 87Z;>"~/9[<;>̺Ϭİn<'QPӉEP xZqFQ꣢xnYSHRH!	yKHVM$-i&^QXYDAz*hӦX)Օ5mիueMx
m9??o{Ҽ>RHAk%-"kgM"T>XHN^ݥ(?:,~,N63 D0ɠ3󯳷,8	PY\T]MT=5PU2?MUls-teF}N<gL:d$&+N|>^l%Ǧx4	tM9+H yÝQtn3OJ~UDWW8$
M4iUPnQp;H{r-iTꕥVVI.)ߛ欭"F^qn5|sýMe}=<4R^oKZ(II+=)dr=;,&
qJvbTE%(<)
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   <Notes>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created>2024.10.12</Created>
<LastModified>2024.10.12</LastModified>
<CreatedBy>L Preston</CreatedBy>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
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ֳvEW_"?"ҡc"0(#)![gp|HVtVxic4V8@	>T|D$Q|a.ŗ!Iډ..H'a̘D4iOݵO:x˟^,;"vΐ|OKRɦU*9mkl o09)ⅾh,f(l+.b"i0_痄Z&Kˡ-8wj6BxEĜvMr<{@XV\07?]_
1xQG(^FX|ZtTL79͋]ū×ְ+O`   θ  vͱg    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
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operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4870-4891" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="M13 rev" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4777-4793" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="17" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" readingFrame="-1" consecutiveTranslationNumbering="1"><Segment range="4801-4817" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="18" name="M13 fwd" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7377-7393" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/></Primers>
  <Notes>
<UUID>41097937-b3b3-47d2-80ff-419e75e8e4b5</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>VB278</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2024.10.11</Created>
<LastModified UTC="7:35:48">2025.8.8</LastModified>
<CreatedBy>L Preston</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  Y     K         Unsaved Enzyme Set                                                                                                    HO  d               E              Y?                                                                                                                                                                <?xml version="1.0"?><EnzymeVisibilities vals="FspI:0,EcoRI:0,XhoI:0,MfeI:0,SacII:0,KpnI:0,EcoRV:0,SpeI:0,SalI:0,ScaI:0,NotI:0,HindIII:0,BamHI:0,NdeI:0,NheI:0"/>
   <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="BamHI EcoRI EcoRV FspI HindIII KpnI MfeI NdeI NheI NotI SacII SalI ScaI SpeI XhoI"/></CustomEnzymeSets>
