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Iame2d~ ` %+ 	Y


	x|Zr ?:I6
s
OTyH Z  gQz


Y	@VJ`i oqq	>T|51Y  P8$	{	40 7i   2sxu5>s
R`RA o=p	
8}F?8  jDf	27?uPASv8  ,p
86~/1`' UeWvJO	
 qC~T>PU  j  A|
`		 1 G snO	~	`Ol[;\m1<e !	Y	k	

 	0(V0' w #/wp		#uUC*34bT! H	
y{/ f$P
*u5


HqG}!   wt<o	
|S4=r4~0@$  TC@uIN	175}.S=T v}	tT'l0d# 
 #Ssn	6|-3Rpk/Sc/   RPS
<;<=>NWXY'(>2\o	
'()  / C  QT
\
=?O,4 )?fgj +?	%~8OZ"]);   U
M>2@gJ,& 0 D P

/{2h!15~L@<h%   w}8R]
P;Y-\*5O98jF M <%!k  )@w	r/F#s?*3A<! ,:&	5mPn$;iY 5   F6, [^e1^:LM  fM		`&k9rL owS		
B5?j'F#   HZV
)	pf,PfH IhcK- $  Nh


JIN!3AXU7=w  E 0Nz
}MM=(   u>Q
r|39f26LJWP 1  !#4S[>0"@AGI  ]b[iBKbiT  3  x^
h

]+T!   ~o FZ.iaC:9$G], \    %AU	LSHQe<a]; +6PyXdk ( K9z

H!tO(|C 6z<l{/	c
=S&b"}Zw @C			?2u@- e  !
 $ldul N %O;d5ut *h5Aq1	6	G#A   _(.x		Mt@=  /;sw qK    g]b0G$s+4B =
6Doj/-Z- dG:K		
/3:#*k|%<Mq6* c x	FM{nS3Xa -;`['	-DQZ+Gp!  bq	C
,W+s_\A 2MkN*  7	Q	-/  
G
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	%C;Y#  / g	
SaoH^ I b		N	y|'lM]:7d #]mT	%l:LCV  |?])	\	r		.
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7DN.c
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*)-q-oI W KM	YgKTiL. K9	hJx  ,k

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OVtk^en  % Y X
	`
JX4x-J>W (( 5  6`?	B		
(C)&BY_A8CQ\x% b1F{% iE		
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/o"*	F' _ 9yr

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>tu F G
~N)zS   B?s4S
q3&)  B O>	R
}{37M1Q[nRb  vO
1:Xg 0$K;iI    jq#.:EKX0K nM
(H<e"vG    "/\Mq8LeAHJ) 2   $@T~
 G\dE` 'Bx  5?;:1t#ks   ^g.p\avF"@ c9	,jCLWFYo 	\i $BLj 	S	
k6cU# s yW(=
%o E,7$!  ,  W+
a^yU   +J
i
wJSg">{"6$ 4 W0vg
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'()^,sV&P[ ^ m8x0=bRPR^X)     N	Rz/p#"-9D:%ZhmHQ#    .mLFcpG_dj;ru  f[`EKV!h#A?n,  VI/V'
n&vIRf=z!%OZ55 ]    eM7K	Ebcy_o/<D!,8Q~@OYgl>mtPW_    &!"TUklmn&J
tuGbwx^7]ef?@<=NY34o- >  'B
#9oYJ

3H%A<^Ai# :$&:Q	z."u=_$  PL'P)naS	4 ) ? (p$	{


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vT=O[] 2	%, S l {%'2.=	d
@{/'>c~ h hM	<_k^vA6T   D(z*Zg.9  QAx
@3QEb
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l

95FJ'mc~ @ E		|


C%'vsE' * <  )c~5"%lrm=pg	m 8 : f 0C,	!e O ]Ub	gR	Ve7rC| JP< M&	(
G
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6j+.xu%% +Xi	
TZfrw$a3 5plo$yM*  1_Z
UnnB1 L^6BWr2	1	7		c$?XQTg_H`1kt7~$ 8!	-Z`GSny			2N^ K )-Q3\ E=K{>  `<<Qf	>J`9 u   e9/Y}	4	I		
wUPA(- }    Iy7jQ
	=		!
Q&^L   <tIr~ 	ip Q f	03Xx" I  V(/B	|1m5" !  u&ie
A bc[v
]>Cg& T p 3h|	
)0^DqH%gt9 m |4*14>CD	d			\~{Nj(?do, >

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6pB0@7, ~N		 		

"_/BxkU.3[/ $ evOHu);	

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;Lz[}N7E+" E(5L	v	_4+lmhdr492@ #  G		
	A4oTe+Ob  RZZ|IF ' [ Kg
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N>R4|Y pLr" w<_F	.CEL2)R{2*,`)  a	P		<
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GF (,|:n!Oy V j-		
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 A N
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's[D&
wd!F o8	R	
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$'`(+]x6# ^"J g": =   H d8j%6I

m%2sFDan;.C\N>;MXhls
n, O%~#&	&

y-hk,ap`Rqvz# 1-				
?<Ni g  ;^j"f
D^VZ 7 9 ; bD	
8E&< y \
>
f*+*% 0^YV	
TbS
Wmf^
0s)} F	H	~1 ,I_8DJz' P | eH6<		 
hP}, o  3U3B{Ahc				MCpZ 6c}	^	
A2.g u	
^}lUc;8e FJ#-	

=rm{HN% ^Ek	O	;
A
KQz(^q() \,^'F'~_U	g		0
-m${Glx  >nM
	vDW , Nt}$	%
)
H
x,&*:g-wo:LW a	

7>;M' { dTAX@;	G			
}lY/y& E~]",	]	t	@

qkT1f&  sE}^f	
/
w9f,unw @\*	+	:	s	


v78Stve  3  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        !                                                                                                                                                                                                                                                                                                                             3                                                                                                                                                      	                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                       	                                                                              	         (                                      	         
                           	                                             6                                                     
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                  "         	                  	         A   !      	                                 	   
         
                  
   
            	            
            	         "   ,   -      '   6         3   %         \          e   |   %          )   k                     !                                                                                                                                                     3                                                                      	                                                                                                                                                                                                                                         	                              	   (              	   
         	                 6                   
          !                           
      "   	      	   A !  	           	 
   
      
 
    	    
    	   " , -  ' 6   3 %   \    e | %    ) k   Q            b ~(ap)dea@A{A	
QhSPp}:L(\=)`	NW|zmI	'u `tW+NW`|P
!1qZPV\z m-6bNwro
3\I#C]
Hu Y,		U	g	]il:	v<}Epd	t]l` #_7
ZyhIamxB@[`%7QO)4rr:RZyhaK/SJv X	I'I'		~.9Ga	/I'%v Y	
8[g'LZyh
" 
9	,]	X
'ZyhmZ`P #_8		7
ZdtOyh(4IpCa	x`u* pUEFF^/

Sf1& /]S K/Sa]	
1
Ba		 1Z`utv YaX		
8[g/'I'	a
R"  WxS|t			


Yev#']@DX	v-R2U" 
Sa
bssPeyb
|5?6[UM	yYlPjKb	v 3=rr	:R 	U_A		7$ =cmI?'* 		zJv.x%3]	U	BkE0	i	B	x0 @~G~	~GjK		
bs+ct,  ')\'F'_0
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#%luOU:sw CLqT*y3H4CVb T!KZ7;; ,Rm|		
vT=O[]                  EFKhsckYY3#jA۴΁;赌%"y2a͆rFeh!dfau)hWuFi'U$TZJ*iXm^IfPq]Zm&ER3v/ @,5|#E3 !"x;Ϫ əHK<E	H"
>p)2>~4:R:POϲ:> ̌[8ROjU1ΪFKV0=Fwjª pج10[ $S#3"l?P,7ҌQο;U0DuAT<{|7i+:I.( 
1*w=:bZZ*+k>
*ڴ"O}P1 81\=ԭ2/@cߜ58+n7N?#?#<}SMǮ/=r7ۮ5Q+1lNPA] NyOu˙M\6!zo=lAL;<}E8~K5Gqm/<6ԇ.Dʕ#뒏On(>#YVVhI  t AuCcz3CK63483ԃwkݽ_KAJ4#Au`RM˷7X4M:ήCP?/6U3rۼ |I3r@s2ǟwݱ0-8^B ,0s,S 0̓ġ0Ã]>3A?2
=.@<;03<?>~?~q0:-,B?3~5?tvFvu}בcHUV:V掦_iY]J]u֍b
B5%_Ո'?SoJZ\Dfb=Rxye&Z&B姦ux'UTDXT)dQZ*YYY]bR%fYWեWv2֩͆rrfn@eZkO,k?!,uʧ\W2ץvNLQt4X1`L3iL 29ޏLL:rë;jHp߱U^!$ªKܮ+]wn溡
UXNUj\Ū]VisZzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|XӃqЫT5ib:cɊ"]jWNasVyPU8'z
k32̷t= Wkr*TT[        '<AdditionalSequenceProperties>
<UpstreamStickiness>0</UpstreamStickiness>
<DownstreamStickiness>0</DownstreamStickiness>
<UpstreamModification>FivePrimePhosphorylated</UpstreamModification>
<DownstreamModification>FivePrimePhosphorylated</DownstreamModification>
</AdditionalSequenceProperties>

  ;<?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="PGK promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2783-3293" color="#66cc99" type="standard"/><Q name="note"><V text="/vntifkey=21"/></Q><Q name="label"><V text="PGK\promoter"/></Q></Feature><Feature recentID="10" name="puro" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3306-3905" color="#66cc99" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="/vntifkey=21"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="label"><V text="puro"/></Q></Feature><Feature recentID="9" name="SV40 pA" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3906-4127" color="#66cc99" type="standard"/><Q name="note"><V text="/vntifkey=21"/></Q><Q name="label"><V text="SV40\pA"/></Q></Feature><Feature recentID="8" name="EF1A promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-1781" color="#66cc99" type="standard"/><Q name="note"><V text="/vntifkey=21"/></Q><Q name="label"><V text="EF1A\promoter"/></Q></Feature><Feature recentID="7" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2507" maxFusedRunOn="2507"><Segment range="1788-2504" color="#66cc99" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="/vntifkey=21"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="label"><V text="EGFP"/></Q></Feature><Feature recentID="6" name="BGH pA" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2551-2775" color="#66cc99" type="standard"/><Q name="note"><V text="/vntifkey=21"/></Q><Q name="label"><V text="BGH\pA"/></Q></Feature><Feature recentID="5" name="pUC ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6427-7015" color="#ef6cdf" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="/application_notes=Facilitates plasmid replication in E.&#10;coli; regulates high-copy plasmid number (500-700)."/><V text="/description=pUC origin of replication"/><V text="/full_name=pUC origin of replication"/><V text="/official_designation=pUC ori"/><V text="/uuid=b978ad4ff785460db448caf63b728237"/></Q><Q name="label"><V text="pUC ori"/></Q></Feature><Feature recentID="4" name="AmpiciIIin" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5396-6256" color="#5566f5" type="standard"/><Q name="note"><V text="/application_notes=Allows E. coli to be resistant to&#10;ampiciIIin."/><V text="/description=AmpiciIIin resistance gene"/><V text="/full_name=Ampicillin resistance gene"/><V text="/official_designation=Ampicillin"/><V text="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"/></Q><Q name="label"><V text="AmpiciIIin"/></Q></Feature><Feature recentID="3" name="3' ITR" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4330-4564" color="#f58600" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 3' inverted terminal repeat"/><V text="/official_designation=3' ITR"/><V text="/uuid=22382df36c4b4754b7821e5d7876f273"/></Q><Q name="label"><V text="3' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="2" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="314-562" color="#512bbd" type="standard"/><Q name="note"><V text="/application_notes=Pol III promoter; drives expression of&#10;small RNAs."/><V text="/description=Human U6 small nuclear 1 promoter"/><V text="/full_name=Human U6 promoter"/><V text="/official_designation=U6"/><V text="/uuid=0f9979e4676b4788b9441e4543b07317"/></Q><Q name="label"><V text="U6 promoter"/></Q></Feature><Feature recentID="1" name="5' ITR" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-313" color="#946a00" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 5' inverted terminal repeat"/><V text="/official_designation=5' ITR"/><V text="/uuid=cf6f8504beb5412ca461e47810e15037"/></Q><Q name="label"><V text="5' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7600" color="#ffffff" type="standard"/></Feature></Features>
   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
   <Notes>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created>2025.8.29</Created>
<LastModified>2025.8.29</LastModified>
<CreatedBy>L Preston</CreatedBy>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
                  EFKhsckYY3#jA۴΁;赌%"y2a͆rFeh!dfau)hWuFi'U$TZJ*iXm^IfPq]Zm&ER3v/ @,5|#E3 !"x;Ϫ əHK<E	H"
>p)2>~4:R:POϲ:> ̌[8ROjU1ΪFKV0=Fwjª pج10[ $S#3"l?P,7ҌQο;U0DuAT<{|7i+:I.( 
1*w=:bZZ*+k>
*ڴ"O}P1 81\=ԭ2/@cߜ58+n7N?#?#<}SMǮ/=r7ۮ5Q+1lNPA] NyOu˙M\6!zo=lAL;<}E8~K5Gqm/<6ԇ.Dʕ#뒏On(>#YVVhI  t AuCcz3CK63483ԃwkݽ_KAJ4#Au`RM˷7X4M:ήCP?/6U3rۼ |I3r@s2ǟwݱ0-8^B ,0s,S 0̓ġ0Ã]>3A?2
=.@<;03<?>~?~q0:-,B?3~5?tvFvu}בcHUV:V掦_iY]J]u֍b
B5%_Ո'?SoJZ\Dfb=Rxye&Z&B姦ux'UTDXT)dQZ*YYY]bR%fYWեWv2֩͆rrfn@eZkO,k?!,uʧ\W2ץvNLQt4X1`L3iL 29ޏLL:rë;jHp߱U^!$ªKܮ+]wn溡
UXNUj\Ū]VisZzX梦gkVbo;aeXYfTIj,6\ITcJ7x	F*QrM+bi&<Dװ͝E'Mp`tE4=He0\l|PN_:7]ؗl8XSqwcM`\5y秹:5֖GR޸੣VOL0hxтSl KC^`aqY8FB>Sh%*{fgzکjU!Fea[\GTmPn3%WUW9
N}{P4LџVx"O$=E$>v~8K"(qP08\ +#wOӠL\~w!9Ts	%0,A7@s*~Lc >`Us\
>b2_&>!8g4q3LKHLALLg5|XӃqЫT5ib:cɊ"]jWNasVyPU8'z
k32̷t= Wkr*TT[        '<AdditionalSequenceProperties>
<UpstreamStickiness>0</UpstreamStickiness>
<DownstreamStickiness>0</DownstreamStickiness>
<UpstreamModification>FivePrimePhosphorylated</UpstreamModification>
<DownstreamModification>FivePrimePhosphorylated</DownstreamModification>
</AdditionalSequenceProperties>

  ;<?xml version="1.0"?><Features nextValidID="12"><Feature recentID="11" name="PGK promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2783-3293" color="#66cc99" type="standard"/><Q name="note"><V text="/vntifkey=21"/></Q><Q name="label"><V text="PGK\promoter"/></Q></Feature><Feature recentID="10" name="puro" directionality="1" translationMW="21497.23" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3306-3905" color="#66cc99" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="/vntifkey=21"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="label"><V text="puro"/></Q></Feature><Feature recentID="9" name="SV40 pA" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3906-4127" color="#66cc99" type="standard"/><Q name="note"><V text="/vntifkey=21"/></Q><Q name="label"><V text="SV40\pA"/></Q></Feature><Feature recentID="8" name="EF1A promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-1781" color="#66cc99" type="standard"/><Q name="note"><V text="/vntifkey=21"/></Q><Q name="label"><V text="EF1A\promoter"/></Q></Feature><Feature recentID="7" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2507" maxFusedRunOn="2507"><Segment range="1788-2504" color="#66cc99" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="/vntifkey=21"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="label"><V text="EGFP"/></Q></Feature><Feature recentID="6" name="BGH pA" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2551-2775" color="#66cc99" type="standard"/><Q name="note"><V text="/vntifkey=21"/></Q><Q name="label"><V text="BGH\pA"/></Q></Feature><Feature recentID="5" name="pUC ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6427-7015" color="#ef6cdf" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="/application_notes=Facilitates plasmid replication in E.&#10;coli; regulates high-copy plasmid number (500-700)."/><V text="/description=pUC origin of replication"/><V text="/full_name=pUC origin of replication"/><V text="/official_designation=pUC ori"/><V text="/uuid=b978ad4ff785460db448caf63b728237"/></Q><Q name="label"><V text="pUC ori"/></Q></Feature><Feature recentID="4" name="AmpiciIIin" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5396-6256" color="#5566f5" type="standard"/><Q name="note"><V text="/application_notes=Allows E. coli to be resistant to&#10;ampiciIIin."/><V text="/description=AmpiciIIin resistance gene"/><V text="/full_name=Ampicillin resistance gene"/><V text="/official_designation=Ampicillin"/><V text="/uuid=6a7994dc58024d80b36e4a85ad2e1d99"/></Q><Q name="label"><V text="AmpiciIIin"/></Q></Feature><Feature recentID="3" name="3' ITR" directionality="2" type="misc_feature" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4330-4564" color="#f58600" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 3' inverted terminal repeat"/><V text="/official_designation=3' ITR"/><V text="/uuid=22382df36c4b4754b7821e5d7876f273"/></Q><Q name="label"><V text="3' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="2" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="314-562" color="#512bbd" type="standard"/><Q name="note"><V text="/application_notes=Pol III promoter; drives expression of&#10;small RNAs."/><V text="/description=Human U6 small nuclear 1 promoter"/><V text="/full_name=Human U6 promoter"/><V text="/official_designation=U6"/><V text="/uuid=0f9979e4676b4788b9441e4543b07317"/></Q><Q name="label"><V text="U6 promoter"/></Q></Feature><Feature recentID="1" name="5' ITR" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-313" color="#946a00" type="standard"/><Q name="note"><V text="/application_notes=Recognized by PBase transposase; DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 5' inverted terminal repeat"/><V text="/official_designation=5' ITR"/><V text="/uuid=cf6f8504beb5412ca461e47810e15037"/></Q><Q name="label"><V text="5' ITR"/><V text="nonstandard type:  ITR"/></Q></Feature><Feature recentID="0" name="source" type="source" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="1-7600" color="#ffffff" type="standard"/></Feature></Features>
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   <Notes>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created>2025.8.29</Created>
<LastModified>2025.8.29</LastModified>
<CreatedBy>L Preston</CreatedBy>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  `7zXZ  ִF !   t/ ] ˇf{3(1v%f M*	mj)p80*i	NY]QF7&!|NnFig6*.wOҪ$FMr{ikbOZ&_"0:*xVxyK*v<}2g#8ۼ]j]tWB@/(cp^/25e#6ݎ1oȄmJc%qyJv"W2tk6p`yty_0OQ@!fUC7,WBo߉J)c提*fۮ)~f[G=iܣ*PiD7ڠB"3eO*j?Eys_w?+/Z+/;#yŐ/bV$P눂PnYfgn	'.u7QF8T
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bF{x|R^R8F-4^#Mi~r}}i	e@q}c=F`Q˵_NCB{@a-_EX"W'4HwR6#oҌ(8UM.#Q˛`8[Ibs>|><mse-S3wF0b3htRp8]_{^9N׭H$``><OONp1x2-ZeI
QU=WHqtY9^":k4yާl9i"[Xmn	>8;,*>[.=)HpW=|_sܥ$s8pȋCܜIE'ڶŌe*tCGD=ޑF|(>aߩjwhM	Ta߱y74g24q\xPoOF&L[`9F^K/t[C"xOkh $	y_Vz 2uK{4˩:^xС`E}%̒Z= I_X^QKi,ilF\	S+ iKr
`Tz#)#}Wo*2,oI	
QEV#_4(%.#|#6ނ+%Lfn8	iFRyILɔ ϶i	!QΩ}!Tg[E	!B>N0XzcqQy)$}jcN3J uHgCL4%u,(c3}B"Q-N9I8zr>0vJ	v5.3˞@+R   TZ-gi ,  Jlg    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="12"><Feature recentID="0" name="NeoR/KanR" directionality="1" translationMW="29006.71" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3306-4100" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (Geneticin®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MAEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="1" name="PGK promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2783-3293" color="#66cc99" type="standard"/><Q name="label"><V text="PGK\promoter"/></Q><Q name="note"><V text="/vntifkey=21"/></Q></Feature><Feature recentID="2" name="SV40 pA" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4101-4322" color="#66cc99" type="standard"/><Q name="label"><V text="SV40\pA"/></Q><Q name="note"><V text="/vntifkey=21"/></Q></Feature><Feature recentID="3" name="EF1A promoter" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="603-1781" color="#66cc99" type="standard"/><Q name="label"><V text="EF1A\promoter"/></Q><Q name="note"><V text="/vntifkey=21"/></Q></Feature><Feature recentID="4" name="EGFP" directionality="1" translationMW="26942.08" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2507" maxFusedRunOn="2507"><Segment range="1788-2504" color="#66cc99" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="label"><V text="EGFP"/></Q><Q name="note"><V text="/vntifkey=21"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"/></Q></Feature><Feature recentID="5" name="BGH pA" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2551-2775" color="#66cc99" type="standard"/><Q name="label"><V text="BGH\pA"/></Q><Q name="note"><V text="/vntifkey=21"/></Q></Feature><Feature recentID="6" name="pUC ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6622-7210" color="#ef6cdf" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="label"><V text="pUC ori"/></Q><Q name="note"><V text="/application_notes=Facilitates plasmid replication in E.&#10;coli; 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DNA&#10;flanked by piggyBac 5' ITR and 3' ITR can be transposed by PBase into TTAA sites."/><V text="/description=piggyBac 3' inverted terminal repeat"/><V text="/official_designation=3' ITR"/><V text="/uuid=22382df36c4b4754b7821e5d7876f273"/></Q></Feature><Feature recentID="9" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="314-562" color="#512bbd" type="standard"/><Q name="label"><V text="U6 promoter"/></Q><Q name="note"><V text="/application_notes=Pol III promoter; drives expression of&#10;small RNAs."/><V text="/description=Human U6 small nuclear 1 promoter"/><V text="/full_name=Human U6 promoter"/><V text="/official_designation=U6"/><V text="/uuid=0f9979e4676b4788b9441e4543b07317"/></Q></Feature><Feature recentID="10" name="5' ITR" directionality="1" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1-313" color="#946a00" type="standard"/><Q name="label"><V text="5' ITR"/><V text="nonstandard type:  ITR"/></Q><Q name="note"><V text="/application_notes=Recognized by PBase transposase; 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   <?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/></Primers>
  a<Notes>
<UUID>90605f93-a0ba-419a-b72a-2341ba317396</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2025.8.28</Created>
<LastModified UTC="5:44:32">2025.8.29</LastModified>
<CreatedBy>L Preston</CreatedBy>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>Unspecified</TransformedInto>
</Notes>
  Y     K         Unsaved Enzyme Set                                                                                                    HO  d               E              Y?                                                                                                                                                                <?xml version="1.0"?><CustomEnzymeSets><CustomEnzymeSet type="2" name="Unsaved Enzyme Set" enzymeNames="BamHI EcoRI EcoRV FspI HindIII KpnI MfeI NdeI NheI NotI SacII SalI ScaI SpeI XhoI"/></CustomEnzymeSets>
