	   SnapGene      $hgcttaatgtagtcttatgcaatactcttgtagtcttgcaacatggtaacgatgagttagcaacatgccttacaaggagagaaaaagcaccgtgcatgccgattggtggaagtaaggtggtacgatcgtgccttattaggaaggcaacagacgggtctgacatggattggacgaaccactgaattgccgcattgcagagatattgtatttaagtgcctagctcgatacataaacgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccagagagggcctatttcccatgattccttcatatttgcatatacgatacaaggctgttagagagataattagaattaatttgactgtaaacacaaagatattagtacaaaatacgtgacgtagaaagtaataatttcttgggtagtttgcagttttaaaattatgttttaaaatggactatcatatgcttaccgtaacttgaaagtatttcgatttcttggctttatatatcttgtggaaaggacgaaacaccggagacggttgtaaatgagcacacaaaatacacatgctaaaatattatattctatgacctttataaaatcaaccaaaatcttctttttaataactttagtatcaataattagaatttttatgttcctttttgcaaacttttaataaaaatgagcaaaataaaaaaacgctagttttagtaactcgcgttgttttcttcacctttaataatagctactccaccacttgttcctaagcggtcagctcctgcttcaatcattttttgagcatcttcaaatgttctaactccaccagctgctttaactaaagcattgtctttaacaactgacttcattagtttaacatcttcaaatgttgcacctgattttgaaaatcctgttgatgttttaacaaattctaatccagcttcaacagctatttcacaagctttcatgatttcttcttttgttaataaacaattttccataatacatttaacaacatgtgatccagctgctttttttacagctttcatgtcttctaaaactaattcataatttttgtcttttaatgcaccaatatttaataccatatcaatttctgttgcaccatctttaattgcttcagaaacttcgaatgcttttgtagctgttgtgcatgcacctagaggaaaacctacaacatttgttattcctacatttgtgccttttaataattctttacaatagcttgttcaatatgaattaacacaaactgttgcaaaatcaaattcaattgcttcatcacataattgtttaatttcagctttcgtagcatcttgttttaataatgtgtgatctatatatttgtttagtttcattttttctcctatatattcatttttaattttaattctttaataatttcgtctactttaactttagcgttttgaacagattcaccaacacctataaaataaatttttagtttaggttcagttccacttgggcgaacagcaaatcatgacttatcttctaaataaaattttagtaagtcttgtcctggcatattatacattccatcgatgtagtcttcaacattaacaactttaagtccagcaatttgagttaagggtgttgctctcaatgatttcattaatggttcaatttttaatttcttttcttctggtttaaaattcaagtttaaagtgaaagtgtaatatgcacccatttctttaaataaatcttctaaatagtctactaatgttttattttgttttttataaaatcaagcagcctctgctattaatatagaagcttgtattccatctttatctctagctgagtcatcaattacatatccataactttcttcataagcaaaaacaaaatttaatccgttatcttcttctttagcaatttctctacccattcatttaaatccagttaaagtttttacaatattaactccatatttttcatgagcgattctatcacccaaatcacttgttacaaaacttgaatatagagccggattttttggaatgctatttaagcgttttagatttgataattttcaatcaattaaaattggtcctgtttgatttccatctaatcttacaaaatgaccatcatgttttattgccattccaaatctgtcagcatctgggtcattcataataataatatctgcatcatgtttaataccatattcaagcggtatttttcatgcaggatcaaattctggatttggatttacaacatttttaaatgtttcatcttcaaatgcatgctcttcaacctcaataacgttatatcctgattcacgtaatatttttggggtaaatttagttcctgttccattaactgcgctaaaaataatttttaaatcttttttagcttcttgctcttttttgtacgtctctgtttaagagctatgctggaaacagcatagcaagtttaaataaggctagtccgttatcaactttgctggaaacagcaaagtggcaccgagtcggtgctttttttgaattctagatcttgagacaaatggcagtattcatccacaattttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagatccactttggcgccggctcgagtggctccggtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtgtcgtgacgcgggatccgccaccatggattacaaagacgatgacgataagatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtagacgcgttaagtcgacaatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcgtcgactttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaagatctgctttttgcttgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtacgtatagtagttcatgtcatcttattattcagtatttataacttgcaaagaaatgaatatcagagagtgagaggaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggctctagctatcccgcccctaactccgcccatcccgcccctaactccgcccagttccgcccattctccgccccatggctgactaattttttttatttatgcagaggccgaggccgcctcggcctctgagctattccagaagtagtgaggaggcttttttggaggcctagggacgtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgcttacaatttaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctgcaa  6L  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,GDGCHC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,GCCGGC,CAATTG,ACGCGT,TCCRAC,GTYGGA,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,RGCR,YGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                                                                                                                                                                                                                                                                                                                                                   /                                                                                                                                                                                                                                                                                                                                                                                                                                                                                         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                                                                                                                                                                                 $                                            	          	                                                                       
                                   	   "            !         	                           5                           
                           	         
         
   	                     
                           (          :   %      $         "            |      *   a   o   N      #   R   t                                                                                                                                                                        /                                                                                                                                                                                                                                                                                               	                      	          
                                                               $                	    	                         
             	 "    !   	         5         
         	   
   
 	       
         (    : %  $   "    |  * a o N  # R t   Bm  C  C  C  H  N{  Z
d"$a IJ$(3 F """i#SDt
2!gk:u I9x"#^#A<\B	

7Y0$-}9EU
6Qo` "#Q###4$Z$6@Ot-StW-	St-S--Dt
<QW"i j `w sJ`7svJ# iE rJr_(#j&3wuX0E}#O#$r&_q#o5	
U,Mo L6
. 0;(A	*"

#!
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9\4n"  <Notes>
<UUID>1ac1c9ad-fa2f-4c12-8191-d0d859bc40c1</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>LRG2.1</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>Lentiviral expression plasmid of sgRNA with GFP.</Description>
<Created UTC="4:0:0">2025.4.14</Created>
<LastModified UTC="4:0:0">2025.4.14</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference journalName="Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 10.1016/j.molcel.2018.02.011." journal="Mol Cell. 2018 Feb 28. pii: S1097-2765(18)30109-6. doi: 10.1016/j.molcel.2018.02.011." authors="Tarumoto Y, Lu B, Somerville TDD, Huang YH, Milazzo JP, Wu XS, Klingbeil O, El Demerdash O, Shi J, Vakoc CR" type="Journal Article" pubMedID="29526696" title="LKB1, Salt-Inducible Kinases, and MEF2C Are Linked Dependencies in Acute Myeloid Leukemia."/>
</References>
</Notes>
  Y              Unique 6+ Cutters                                                                                                     HO  d                E             Y?                                                                                                                                                               9<?xml version="1.0"?><Primers recycledIDs="0,2,3,4,5,6,7,8,9,11,17,18,19,20,21,22,23,25,26,27,28,30,31,33,34,39,40,41,42,43,44,45,46,47,48,49,50,51,52,53,55,56,57,58,59,60,61,62,63,64,65,66,67,68,69,70,71,72,73,74,79,80,81,76" nextValidID="83"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="1" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="7455-7474" boundStrand="1" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="7455-7474" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="7455-7474" boundStrand="1" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="7455-7474" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="10" name="EBV-rev" sequence="GTGGTTTGTCCAAACTCATC" description="SV40 polyA terminator, reverse primer"><BindingSite location="6239-6258" boundStrand="0" annealedBases="GTGGTTTGTCCAAACTCATC" meltingTemperature="55"><Component hybridizedRange="6239-6258" bases="GTGGTTTGTCCAAACTCATC"/></BindingSite><BindingSite simplified="1" location="6239-6258" boundStrand="0" annealedBases="GTGGTTTGTCCAAACTCATC" meltingTemperature="55"><Component hybridizedRange="6239-6258" bases="GTGGTTTGTCCAAACTCATC"/></BindingSite></Primer><Primer recentID="12" name="EGFP-C" sequence="CATGGTCCTGCTGGAGTTCGTG" description="EGFP, forward primer"><BindingSite location="5095-5116" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="5095-5116" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite><BindingSite simplified="1" location="5095-5116" boundStrand="0" annealedBases="CATGGTCCTGCTGGAGTTCGTG" meltingTemperature="61"><Component hybridizedRange="5095-5116" bases="CATGGTCCTGCTGGAGTTCGTG"/></BindingSite></Primer><Primer recentID="13" name="EGFP-N" sequence="CGTCGCCGTCCAGCTCGACCAG" description="EGFP, reverse primer"><BindingSite location="4487-4508" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="4487-4508" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite><BindingSite simplified="1" location="4487-4508" boundStrand="1" annealedBases="CGTCGCCGTCCAGCTCGACCAG" meltingTemperature="67"><Component hybridizedRange="4487-4508" bases="CGTCGCCGTCCAGCTCGACCAG"/></BindingSite></Primer><Primer recentID="14" name="EXFP-R" sequence="GTCTTGTAGTTGCCGTCGTC" description="For distinguishing EGFP variants, reverse primer"><BindingSite location="4748-4767" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="4748-4767" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite><BindingSite simplified="1" location="4748-4767" boundStrand="1" annealedBases="GTCTTGTAGTTGCCGTCGTC" meltingTemperature="57"><Component hybridizedRange="4748-4767" bases="GTCTTGTAGTTGCCGTCGTC"/></BindingSite></Primer><Primer recentID="15" name="F1ori-F" sequence="GTGGACTCTTGTTCCAAACTGG" description="F1 origin, forward primer"><BindingSite location="6948-6969" boundStrand="0" annealedBases="GTGGACTCTTGTTCCAAACTGG" meltingTemperature="57"><Component hybridizedRange="6948-6969" bases="GTGGACTCTTGTTCCAAACTGG"/></BindingSite><BindingSite location="8609-8618" boundStrand="1" annealedBases="TCCAACTGG" meltingTemperature="13"><Component bases="GTGGACTCTTGT"/><Component hybridizedRange="8614-8618" bases="TCCAA"/><Component bases="A"/><Component hybridizedRange="8609-8612" bases="CTGG"/></BindingSite><BindingSite simplified="1" location="6948-6969" boundStrand="0" annealedBases="GTGGACTCTTGTTCCAAACTGG" meltingTemperature="57"><Component hybridizedRange="6948-6969" bases="GTGGACTCTTGTTCCAAACTGG"/></BindingSite><BindingSite simplified="1" location="8609-8618" boundStrand="1" annealedBases="TCCAACTGG" meltingTemperature="13"><Component bases="GTGGACTCTTGT"/><Component hybridizedRange="8614-8618" bases="TCCAA"/><Component bases="A"/><Component hybridizedRange="8609-8612" bases="CTGG"/></BindingSite></Primer><Primer recentID="16" name="F1ori-R" sequence="AGGGAAGAAAGCGAAAGGAG" description="F1 origin, reverse primer"><BindingSite location="6738-6757" boundStrand="1" annealedBases="AGGGAAGAAAGCGAAAGGAG" meltingTemperature="57"><Component hybridizedRange="6738-6757" bases="AGGGAAGAAAGCGAAAGGAG"/></BindingSite><BindingSite simplified="1" location="6738-6757" boundStrand="1" annealedBases="AGGGAAGAAAGCGAAAGGAG" meltingTemperature="57"><Component hybridizedRange="6738-6757" bases="AGGGAAGAAAGCGAAAGGAG"/></BindingSite></Primer><Primer recentID="24" name="hU6-F" sequence="GAGGGCCTATTTCCCATGATT" description="Human U6 promoter, forward primer"><BindingSite location="1718-1738" boundStrand="0" annealedBases="GAGGGCCTATTTCCCATGATT" meltingTemperature="56"><Component hybridizedRange="1718-1738" bases="GAGGGCCTATTTCCCATGATT"/></BindingSite><BindingSite location="8932-8941" boundStrand="0" annealedBases="TCCCTGATT" meltingTemperature="12"><Component bases="GAGGGCCTATT"/><Component hybridizedRange="8932-8935" bases="TCCC"/><Component bases="A"/><Component hybridizedRange="8937-8941" bases="TGATT"/></BindingSite><BindingSite simplified="1" location="1718-1738" boundStrand="0" annealedBases="GAGGGCCTATTTCCCATGATT" meltingTemperature="56"><Component hybridizedRange="1718-1738" bases="GAGGGCCTATTTCCCATGATT"/></BindingSite><BindingSite simplified="1" location="8932-8941" boundStrand="0" annealedBases="TCCCTGATT" meltingTemperature="12"><Component bases="GAGGGCCTATT"/><Component hybridizedRange="8932-8935" bases="TCCC"/><Component bases="A"/><Component hybridizedRange="8937-8941" bases="TGATT"/></BindingSite></Primer><Primer recentID="29" name="L4440" sequence="AGCGAGTCAGTGAGCGAG" description="L4440 vector, forward primer"><BindingSite location="9010-9027" boundStrand="0" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="9010-9027" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite><BindingSite simplified="1" location="9010-9027" boundStrand="0" annealedBases="AGCGAGTCAGTGAGCGAG" meltingTemperature="58"><Component hybridizedRange="9010-9027" bases="AGCGAGTCAGTGAGCGAG"/></BindingSite></Primer><Primer recentID="32" name="LKO.1 5'" sequence="GACTATCATATGCTTACCGT" description="Human U6 promoter, forward primer"><BindingSite location="1889-1908" boundStrand="0" annealedBases="GACTATCATATGCTTACCGT" meltingTemperature="51"><Component hybridizedRange="1889-1908" bases="GACTATCATATGCTTACCGT"/></BindingSite><BindingSite location="5287-5296" boundStrand="0" annealedBases="TGCTTCCGT" meltingTemperature="29"><Component bases="GACTATCATA"/><Component hybridizedRange="5287-5291" bases="TGCTT"/><Component bases="A"/><Component hybridizedRange="5293-5296" bases="CCGT"/></BindingSite><BindingSite simplified="1" location="1889-1908" boundStrand="0" annealedBases="GACTATCATATGCTTACCGT" meltingTemperature="51"><Component hybridizedRange="1889-1908" bases="GACTATCATATGCTTACCGT"/></BindingSite><BindingSite simplified="1" location="5287-5296" boundStrand="0" annealedBases="TGCTTCCGT" meltingTemperature="29"><Component bases="GACTATCATA"/><Component hybridizedRange="5287-5291" bases="TGCTT"/><Component bases="A"/><Component hybridizedRange="5293-5296" bases="CCGT"/></BindingSite></Primer><Primer recentID="35" name="M13 Forward" sequence="TGTAAAACGACGGCCAGT" description="In lacZ gene. Also called M13-F20 or M13 (-21) Forward"><BindingSite location="6493-6510" boundStrand="1" annealedBases="TGTAAAACGACGGCCAGT" meltingTemperature="57"><Component hybridizedRange="6493-6510" bases="TGTAAAACGACGGCCAGT"/></BindingSite><BindingSite simplified="1" location="6493-6510" boundStrand="1" annealedBases="TGTAAAACGACGGCCAGT" meltingTemperature="57"><Component hybridizedRange="6493-6510" bases="TGTAAAACGACGGCCAGT"/></BindingSite></Primer><Primer recentID="36" name="M13 Reverse" sequence="CAGGAAACAGCTATGAC" description="In lacZ gene. Also called M13-rev"><BindingSite location="9242-9258" boundStrand="0" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="9242-9258" bases="CAGGAAACAGCTATGAC"/></BindingSite><BindingSite simplified="1" location="9242-9258" boundStrand="0" annealedBases="CAGGAAACAGCTATGAC" meltingTemperature="49"><Component hybridizedRange="9242-9258" bases="CAGGAAACAGCTATGAC"/></BindingSite></Primer><Primer recentID="37" name="M13/pUC Forward" sequence="CCCAGTCACGACGTTGTAAAACG" description="In lacZ gene"><BindingSite location="6502-6524" boundStrand="1" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6502-6524" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite><BindingSite simplified="1" location="6502-6524" boundStrand="1" annealedBases="CCCAGTCACGACGTTGTAAAACG" meltingTemperature="61"><Component hybridizedRange="6502-6524" bases="CCCAGTCACGACGTTGTAAAACG"/></BindingSite></Primer><Primer recentID="38" name="M13/pUC Reverse" sequence="AGCGGATAACAATTTCACACAGG" description="In lacZ gene"><BindingSite location="9223-9245" boundStrand="0" annealedBases="AGCGGATAACAATTTCACACAGG" meltingTemperature="58"><Component hybridizedRange="9223-9245" bases="AGCGGATAACAATTTCACACAGG"/></BindingSite><BindingSite simplified="1" location="9223-9245" boundStrand="0" annealedBases="AGCGGATAACAATTTCACACAGG" meltingTemperature="58"><Component hybridizedRange="9223-9245" bases="AGCGGATAACAATTTCACACAGG"/></BindingSite></Primer><Primer recentID="54" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="8757-8776" boundStrand="0" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="8757-8776" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="8757-8776" boundStrand="0" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="8757-8776" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="75" name="SV40pA-R" sequence="GAAATTTGTGATGCTATTGC" description="SV40 polyA, reverse primer"><BindingSite location="1216-1226" boundStrand="1" annealedBases="GATGCTTTGC" meltingTemperature="21"><Component bases="GAAATTTGT"/><Component hybridizedRange="1221-1226" bases="GATGCT"/><Component bases="A"/><Component hybridizedRange="1216-1219" bases="TTGC"/></BindingSite><BindingSite location="5280-5289" boundStrand="0" annealedBases="ATGCTATTGC" meltingTemperature="32"><Component bases="GAAATTTGTG"/><Component hybridizedRange="5280-5289" bases="ATGCTATTGC"/></BindingSite><BindingSite location="6185-6204" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="52"><Component hybridizedRange="6185-6204" bases="GAAATTTGTGATGCTATTGC"/></BindingSite><BindingSite simplified="1" location="1216-1226" boundStrand="1" annealedBases="GATGCTTTGC" meltingTemperature="21"><Component bases="GAAATTTGT"/><Component hybridizedRange="1221-1226" bases="GATGCT"/><Component bases="A"/><Component hybridizedRange="1216-1219" bases="TTGC"/></BindingSite><BindingSite simplified="1" location="5280-5289" boundStrand="0" annealedBases="ATGCTATTGC" meltingTemperature="32"><Component bases="GAAATTTGTG"/><Component hybridizedRange="5280-5289" bases="ATGCTATTGC"/></BindingSite><BindingSite simplified="1" location="6185-6204" boundStrand="1" annealedBases="GAAATTTGTGATGCTATTGC" meltingTemperature="52"><Component hybridizedRange="6185-6204" bases="GAAATTTGTGATGCTATTGC"/></BindingSite></Primer><Primer recentID="77" name="T3" sequence="GCAATTAACCCTCACTAAAGG" description="T3 promoter, forward primer"><BindingSite location="6465-6475" boundStrand="1" annealedBases="CTCACTAAGG" meltingTemperature="18"><Component bases="GCAATTAACC"/><Component hybridizedRange="6469-6475" bases="CTCACTA"/><Component bases="A"/><Component hybridizedRange="6465-6467" bases="AGG"/></BindingSite><BindingSite location="9277-9297" boundStrand="0" annealedBases="GCAATTAACCCTCACTAAAGG" meltingTemperature="53"><Component hybridizedRange="9277-9297" bases="GCAATTAACCCTCACTAAAGG"/></BindingSite><BindingSite simplified="1" location="6465-6475" boundStrand="1" annealedBases="CTCACTAAGG" meltingTemperature="18"><Component bases="GCAATTAACC"/><Component hybridizedRange="6469-6475" bases="CTCACTA"/><Component bases="A"/><Component hybridizedRange="6465-6467" bases="AGG"/></BindingSite><BindingSite simplified="1" location="9277-9297" boundStrand="0" annealedBases="GCAATTAACCCTCACTAAAGG" meltingTemperature="53"><Component hybridizedRange="9277-9297" bases="GCAATTAACCCTCACTAAAGG"/></BindingSite></Primer><Primer recentID="78" name="T7" sequence="TAATACGACTCACTATAGGG" description="T7 promoter, forward primer"><BindingSite location="6464-6483" boundStrand="1" annealedBases="TAATACGACTCACTATAGGG" meltingTemperature="50"><Component hybridizedRange="6464-6483" bases="TAATACGACTCACTATAGGG"/></BindingSite><BindingSite location="9287-9298" boundStrand="0" annealedBases="CTCACTAAGGG" meltingTemperature="28"><Component bases="TAATACGA"/><Component hybridizedRange="9287-9293" bases="CTCACTA"/><Component bases="T"/><Component hybridizedRange="9295-9298" bases="AGGG"/></BindingSite><BindingSite simplified="1" location="6464-6483" boundStrand="1" annealedBases="TAATACGACTCACTATAGGG" meltingTemperature="50"><Component hybridizedRange="6464-6483" bases="TAATACGACTCACTATAGGG"/></BindingSite><BindingSite simplified="1" location="9287-9298" boundStrand="0" annealedBases="CTCACTAAGGG" meltingTemperature="28"><Component bases="TAATACGA"/><Component hybridizedRange="9287-9293" bases="CTCACTA"/><Component bases="T"/><Component hybridizedRange="9295-9298" bases="AGGG"/></BindingSite></Primer><Primer recentID="82" name="WPRE-R" sequence="CATAGCGTAAAAGGAGCAACA" description="WPRE, reverse primer"><BindingSite location="1294-1305" boundStrand="0" annealedBases="AAAGGACAACA" meltingTemperature="26"><Component bases="CATAGCGTA"/><Component hybridizedRange="1294-1299" bases="AAAGGA"/><Component bases="G"/><Component hybridizedRange="1301-1305" bases="CAACA"/></BindingSite><BindingSite location="5230-5250" boundStrand="1" annealedBases="CATAGCGTAAAAGGAGCAACA" meltingTemperature="55"><Component hybridizedRange="5230-5250" bases="CATAGCGTAAAAGGAGCAACA"/></BindingSite><BindingSite simplified="1" location="1294-1305" boundStrand="0" annealedBases="AAAGGACAACA" meltingTemperature="26"><Component bases="CATAGCGTA"/><Component hybridizedRange="1294-1299" bases="AAAGGA"/><Component bases="G"/><Component hybridizedRange="1301-1305" bases="CAACA"/></BindingSite><BindingSite simplified="1" location="5230-5250" boundStrand="1" annealedBases="CATAGCGTAAAAGGAGCAACA" meltingTemperature="55"><Component hybridizedRange="5230-5250" bases="CATAGCGTAAAAGGAGCAACA"/></BindingSite></Primer></Primers>

  6<?xml version="1.0"?><Features nextValidID="28"><Feature recentID="0" name="M13 rev" directionality="1" type="primer_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9243-9259" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="M13 fwd" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6494-6510" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5648" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="5574" maxFusedRunOn="5574" detectionMode="exactProteinMatch"><Segment range="5649-5660" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature><Feature recentID="3" name="gp41 peptide" directionality="1" translationMW="1816.04" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="1832" maxFusedRunOn="1832" detectionMode="exactProteinMatch"><Segment range="1497-1541" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;recognized by the 2H10 single-chain llama nanobody&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/23505368&quot;&gt;Lutje Hulsik et al., 2013&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="KNEQELLELDKWASL"/></Q></Feature><Feature recentID="4" name="FLAG" directionality="1" translationMW="1012.98" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5162" maxFusedRunOn="5162" detectionMode="exactProteinMatch"><Segment range="4419-4442" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;FLAG® epitope tag, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDDDDK"/></Q></Feature><Feature recentID="5" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3993-4110" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1079-1312" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="HIV-1 Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="461-586" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="3' LTR (ΔU3)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5838-6071" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;self-inactivating&amp;nbsp;3' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="234-414" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5178-5766" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="U6 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1719-1967" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;RNA polymerase III promoter for human U6 snRNA (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC154217/&quot;&gt;Domitrovich &amp;amp; Kunkel, 2003&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6149-6270" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6310-6445" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="EF-1α core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4179-4390" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1α&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="RSV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7-233" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Rous sarcoma virus enhancer/promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" maxRunOn="5162" maxFusedRunOn="5162" detectionMode="exactProteinMatch"><Segment range="4443-4445" color="#05fc14" type="standard" translated="1"/><Segment name="1a" range="4446-4448" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="4449-5159" color="#05fc14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;enhanced GFP&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"/></Q></Feature><Feature recentID="17" name="EGFP" directionality="1" translationMW="26941.53" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="0" hitsStopCodon="1"><Segment range="4443-4445" color="#05fd14" type="standard" translated="1"/><Segment name="1a" range="4446-4448" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="0"/><Segment range="4449-5162" color="#05fd14" type="standard" translated="1" translationNumberingStartsFrom="2"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;the original enhanced GFP (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/8948654&quot;&gt;Yang et al., 1996&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK*"/></Q></Feature><Feature recentID="18" name="T3 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9280-9298" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T3 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="T7 promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6466-6484" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="20" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4410-4419" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="21" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6652-7107" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="22" name="ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8269-8857" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="lac operator" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9219-9235" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="CAP binding site" type="protein_bind" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="9145-9166" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="lac promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment name="-35" range="9181-9186" color="#ffffff" type="standard"/><Segment range="9187-9204" color="#ffffff" type="standard"/><Segment name="-10" range="9205-9211" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="AmpR" directionality="1" translationMW="31557.36" type="CDS" allowSegmentOverlaps="0" cleavageArrows="7306" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment name="signal sequence" range="7238-7306" color="#ccffcc" type="standard" translated="1"/><Segment range="7307-8098" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="27" name="AmpR promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7133-7237" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   )<?xml version="1.0"?><CustomEnzymeSets/>
