	   SnapGene      "gctccctgcttgtgtgttggaggtcgctgagtagtgcgcgagcaaaatttaagctacaacaaggcaaggcttgaccgacaattgcatgaagaatctgcttagggttaggcgttttgcgctgcttcgcgatgtacgggccagatatacgcgttgacattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtacgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttatgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacatcaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaacaactccgccccattgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagcgcgttttgcctgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagtggcgcccgaacagggacttgaaagcgaaagggaaaccagaggagctctctcgacgcaggactcggcttgctgaagcgcgcacggcaagaggcgaggggcggcgactggtgagtacgccaaaaattttgactagcggaggctagaaggagagagatgggtgcgagagcgtcagtattaagcgggggagaattagatcgcgatgggaaaaaattcggttaaggccagggggaaagaaaaaatataaattaaaacatatagtatgggcaagcagggagctagaacgattcgcagttaatcctggcctgttagaaacatcagaaggctgtagacaaatactgggacagctacaaccatcccttcagacaggatcagaagaacttagatcattatataatacagtagcaaccctctattgtgtgcatcaaaggatagagataaaagacaccaaggaagctttagacaagatagaggaagagcaaaacaaaagtaagaccaccgcacagcaagcggccggccgctgatcttcagacctggaggaggagatatgagggacaattggagaagtgaattatataaatataaagtagtaaaaattgaaccattaggagtagcacccaccaaggcaaagagaagagtggtgcagagagaaaaaagagcagtgggaataggagctttgttccttgggttcttgggagcagcaggaagcactatgggcgcagcgtcaatgacgctgacggtacaggccagacaattattgtctggtatagtgcagcagcagaacaatttgctgagggctattgaggcgcaacagcatctgttgcaactcacagtctggggcatcaagcagctccaggcaagaatcctggctgtggaaagatacctaaaggatcaacagctcctggggatttggggttgctctggaaaactcatttgcaccactgctgtgccttggaatgctagttggagtaataaatctctggaacagatttggaatcacacgacctggatggagtgggacagagaaattaacaattacacaagcttaatacactccttaattgaagaatcgcaaaaccagcaagaaaagaatgaacaagaattattggaattagataaatgggcaagtttgtggaattggtttaacataacaaattggctgtggtatataaaattattcataatgatagtaggaggcttggtaggtttaagaatagtttttgctgtactttctatagtgaatagagttaggcagggatattcaccattatcgtttcagacccacctcccaaccccgaggggacccgacaggcccgaaggaatagaagaagaaggtggagagagagacagagacagatccattcgattagtgaacggatcggcactgcgtgcgccaattctgcagacaaatggcagtattcatccacaattttaaaagaaaaggggggattggggggtacagtgcaggggaaagaatagtagacataatagcaacagacatacaaactaaagaattacaaaaacaaattacaaaaattcaaaattttcgggtttattacagggacagcagagatccagtttggttagtaccgggcccgctctagagatccgacgcgccatctctaggcccgcgccggccccctcgcacggacttgtgggagaagctcggctactcccctgccccggttaatttgcatataatatttcctagtaactatagaggcttaatgtgcgataaaagacagataatctgttctttttaatactagctacattttacatgataggcttggatttctataacttcgtatagcatacattatacgaagttataaacagcacaaaaggaaactcaccctaactgtaaagtaattgtgtgttttgagactataagtatcccttggagaaccaccttgttggaccaggatgggcaccacccgtttaagagctaagctggaaacagcatagcaagtttaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctttttttctcgagtactaggatccattaggcggccgcgtggataaccgtattaccgccatgcatgtgcccgtcagtgggcagagcgcacatcgcccacagtccccgagaagttggggggaggggtcggcaattgaaccggtgcctagagaaggtggcgcggggtaaactgggaaagtgatgtcgtgtactggctccgcctttttcccgagggtgggggagaaccgtatataagtgcagtagtcgccgtgaacgttctttttcgcaacgggtttgccgccagaacacaggtaagtgccgtgtgtggttcccgcgggcctggcctctttacgggttatggcccttgcgtgccttgaattacttccacctggctgcagtacgtgattcttgatcccgagcttcgggttggaagtgggtgggagagttcgaggccttgcgcttaaggagccccttcgcctcgtgcttgagttgaggcctggcctgggcgctggggccgccgcgtgcgaatctggtggcaccttcgcgcctgtctcgctgctttcgataagtctctagccatttaaaatttttgatgacctgctgcgacgctttttttctggcaagatagtcttgtaaatgcgggccaagatctgcacactggtatttcggtttttggggccgcgggcggcgacggggcccgtgcgtcccagcgcacatgttcggcgaggcggggcctgcgagcgcggccaccgagaatcggacgggggtagtctcaagctggccggcctgctctggtgcctggcctcgcgccgccgtgtatcgccccgccctgggcggcaaggctggcccggtcggcaccagttgcgtgagcggaaagatggccgcttcccggccctgctgcagggagctcaaaatggaggacgcggcgctcgggagagcgggcgggtgagtcacccacacaaaggaaaagggcctttccgtcctcagccgtcgcttcatgtgactccacggagtaccgggcgccgtccaggcacctcgattagttctcgcgcttttggagtacgtcgtctttaggttggggggaggggttttatgcgatggagtttccccacactgagtgggtggagactgaagttaggccagcttggcacttgatgtaattctccttggaatttgccctttttgagtttggatcttggttcattctcaagcctcagacagtggttcaaagtttttttcttccatttcaggtgtcgtgagctagcccaccatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgccggatcgggagagggcagaggaagtctgctaacatgcggtgacgtcgaggagaatcctggcccaccggtcgccaccatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgcaggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtaagaattcgtcgagggacctaataacttcgtatagcatacattatacgaagttatacatgtttaagggttccggttccactaggtacaattcgatatcaagcttatcgataatcaacctctggattacaaaatttgtgaaagattgactggtattcttaactatgttgctccttttacgctatgtggatacgctgctttaatgcctttgtatcatgctattgcttcccgtatggctttcattttctcctccttgtataaatcctggttgctgtctctttatgaggagttgtggcccgttgtcaggcaacgtggcgtggtgtgcactgtgtttgctgacgcaacccccactggttggggcattgccaccacctgtcagctcctttccgggactttcgctttccccctccctattgccacggcggaactcatcgccgcctgccttgcccgctgctggacaggggctcggctgttgggcactgacaattccgtggtgttgtcggggaaatcatcgtcctttccttggctgctcgcctgtgttgccacctggattctgcgcgggacgtccttctgctacgtcccttcggccctcaatccagcggaccttccttcccgcggcctgctgccggctctgcggcctcttccgcgtcttcgccttcgccctcagacgagtcggatctccctttgggccgcctccccgcatcgataccgtcgacctcgatcgagacctagaaaaacatggagcaatcacaagtagcaatacagcagctaccaatgctgattgtgcctggctagaagcacaagaggaggaggaggtgggttttccagtcacacctcaggtacctttaagaccaatgacttacaaggcagctgtagatcttagccactttttaaaagaaaaggggggactggaagggctaattcactcccaacgaagacaagatatccttgatctgtggatctaccacacacaaggctacttccctgattggcagaactacacaccagggccagggatcagatatccactgacctttggatggtgctacaagctagtaccagttgagcaagagaaggtagaagaagccaatgaaggagagaacacccgcttgttacaccctgtgagcctgcatgggatggatgacccggagagagaagtattagagtggaggtttgacagccgcctagcatttcatcacatggcccgagagctgcatccggactgtactgggtctctctggttagaccagatctgagcctgggagctctctggctaactagggaacccactgcttaagcctcaataaagcttgccttgagtgcttcaagtagtgtgtgcccgtctgttgtgtgactctggtaactagagatccctcagacccttttagtcagtgtggaaaatctctagcagcatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtcgacggatcgggagatctcccgatcccctatggtgcactctcagtacaatctgctctgatgccgcatagttaagccagtatct  :  GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,GDGCHC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,GCCGGC,CAATTG,ACGCGT,TCCRAC,GTYGGA,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,RGCR,YGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        0                                                                                                                                                                                            	                                                                                                                                9                                                       
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        	                                        	                                              
                                                                         
      4                              ,                   	      	                     
                                         ,                                                    	                                                                                                            #   	         +      
   
   	            	         F   5                                             
                                       	            
   '                  +   ?   -      *   2      +   B   #               h         |      )   %   5   #   t                     0                                                                                        	                                                            9                         
                                                                                                                                                                                                                                          	              	                  
                           
  4          ,       	  	       
               ,                  	                                    # 	   +  
 
 	    	   F 5               
             	    
 '      + ? -  * 2  + B #     h   |  ) % 5 # t   @  Bu  B  B  H  P  ]'	' 	
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VWb`-|x'+6^p-Lm " o!
  up;=]!nI	l  u1@d		[
t=nJSpw;h+yB<H}=+:w|	m
mt|	m
t|	m
tLb !uiL!_~dk"	gw"dgc"H"<H` "zO'L;E	l8 1r!DMD
4_ Uba"g	d" x*zmx89R	j"!!Z})%\O , s		O
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h2Wkm8RB 6r"x!	
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Wg`y<5Zl0[4
<0a)4n~!V	rF):  { f"<`JVJ!z%  <			
zOS{^{0<5<1]9`y<l0<N w		n6#0<<!M!r"} hy3T 'L	p <07	UECV
!pK(d*yF
] u![	N7Z ^d":f0 ng	,AEW^pf!d""{ f8s	U	XHg)D7!"m"w"
<N 
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Iu ZoHK9.Kl   			
r()M<Kz}$z}DU3 	2
j5OND #O !
)w}q6!K$U3 ZoH.Kl u K9	(k <j&;6[Eon4}Mpu ZotwY;(lHHK}29E|.gKzQ l  !" 	%	2
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Ir0ZQ  		|=V/V'J bZ Ahd&\_i	#O4[c"f"~
	g0cl*mz8 C  "IAb'+Z4j. 2[A!  "IRuFw+ ?WC. <foO
(Z|PV#f  [!W zq@HKT3&!t!!&iAh\_f(Z|PV Z dtwY;(lH}2E|gzQ  !"'4L	T	5(9U p U 8O :+	U"6ws
b!h"dCT $ |   g		&yX.=~1Q21P| D"  			Lux<}>PW~0O}h{)
eR  D!!C"":(V'JY />Dbh(lHq"2E$  " %jQ		C gq! "3 ;gA!

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`_lH=:xd!			vko7$1= ; =!N!r!k"s"{"  		
5`es)N[~=z*&]~94G{}\gy+EP 	
3
jS5PN=E> T 
=Wh#8J6zO
  b  "3 C    0;-gA!&L		

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Z


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*JOz$k!7DIVmx 8CYag
'/krx0GJqR2p!-nJMU`=B-J # k n   !-!y!!!!!!"  _ t w  
@t*?HX|5OT^f			 
/
M|<MTmvRo	:Qp3;?S<!MTt7Xj|8;X^~i*DUvV[f     !E!!Y""""T       08')h.0<rl	{	?
A
T






Lkm=Wh#8Jo6zOL	gik
  b h u    !""""!-Vc\"!b"R E"G	W	=
sFiG4 Z s g!"   	%		
-
3
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l^&9tY			
3
#B1Fcho|jvRIS5F\kPEa*N=XEj>  !!"- O  _t"5	4					9

%PcGyx 2|:	
OH|.M]ctBaIa(h6   +wJX
 Y3^G%Nou{ A %\0  -R{X8	7
]

6V?jFDIWw!" 3 !-Vc;gA!T

;@ z{S]$L\C&1J\s1A>E&0]#O[n}i~8* "!]"b"1~]gm?(7V!e"  <Notes>
<UUID>dd962ed8-8d35-4d34-b0ac-809609a1346e</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pMCB320</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Description>3rd generation lentiviral sgRNA expression vector with mCherry, puromycin resistance, BlpI+BstXI cloning sites.</Description>
<Created UTC="4:0:0">2025.4.14</Created>
<LastModified UTC="4:0:0">2025.4.14</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
<References>
<Reference journalName="Nat Biotechnol. 2017 Mar 20. doi: 10.1038/nbt.3834." authors="Han K, Jeng EE, Hess GT, Morgens DW, Li A, Bassik MC" type="Journal Article" title="Synergistic drug combinations for cancer identified in a CRISPR screen for pairwise genetic interactions." pubMedID="28319085" journal="Nat Biotechnol. 2017 Mar 20. doi: 10.1038/nbt.3834."/>
</References>
</Notes>
  Y              Unique 6+ Cutters                                                                                                     HO  d                 E             Y?                                                                                                                                                               *<?xml version="1.0"?><Primers recycledIDs="0,2,3,4,5,7,10,12,13,14,15,16,17,18,19,20,21,22,23,24,25,26,27,28,29,30,31,32,33,34,35,36,37,38,39,42,43,45,46,47,48,49,50,52,53,55,56,57,58,59,60,61,62,63,64,65,66,67,68,72,73,74,75,76,77,78,79,80,81,8,9" nextValidID="83"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40" showAdditionalFivePrimeMatches="1" minimumFivePrimeAnnealing="15"/><Primer recentID="1" name="Amp-R" sequence="ATAATACCGCGCCACATAGC" description="Ampicillin resistance gene, reverse primer"><BindingSite location="8433-8452" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="8433-8452" bases="ATAATACCGCGCCACATAGC"/></BindingSite><BindingSite simplified="1" location="8433-8452" boundStrand="0" annealedBases="ATAATACCGCGCCACATAGC" meltingTemperature="58"><Component hybridizedRange="8433-8452" bases="ATAATACCGCGCCACATAGC"/></BindingSite></Primer><Primer recentID="6" name="CMV-F" sequence="CGCAAATGGGCGGTAGGCGTG" description="Human CMV immediate early promoter, forward primer"><BindingSite location="686-706" boundStrand="0" annealedBases="CGCAAATGGGCGGTAGGCGTG" meltingTemperature="65"><Component hybridizedRange="686-706" bases="CGCAAATGGGCGGTAGGCGTG"/></BindingSite><BindingSite simplified="1" location="686-706" boundStrand="0" annealedBases="CGCAAATGGGCGGTAGGCGTG" meltingTemperature="65"><Component hybridizedRange="686-706" bases="CGCAAATGGGCGGTAGGCGTG"/></BindingSite></Primer><Primer recentID="11" name="EF1a-F" sequence="TCAAGCCTCAGACAGTGGTTC" description="Human elongation factor-1a promoter, forward primer"><BindingSite location="4137-4157" boundStrand="0" annealedBases="TCAAGCCTCAGACAGTGGTTC" meltingTemperature="58"><Component hybridizedRange="4137-4157" bases="TCAAGCCTCAGACAGTGGTTC"/></BindingSite><BindingSite simplified="1" location="4137-4157" boundStrand="0" annealedBases="TCAAGCCTCAGACAGTGGTTC" meltingTemperature="58"><Component hybridizedRange="4137-4157" bases="TCAAGCCTCAGACAGTGGTTC"/></BindingSite></Primer><Primer recentID="40" name="mCherry-F" sequence="CCCCGTAATGCAGAAGAAGA" description="mCherry, forward primer"><BindingSite location="5286-5305" boundStrand="0" annealedBases="CCCCGTAATGCAGAAGAAGA" meltingTemperature="54"><Component hybridizedRange="5286-5305" bases="CCCCGTAATGCAGAAGAAGA"/></BindingSite><BindingSite simplified="1" location="5286-5305" boundStrand="0" annealedBases="CCCCGTAATGCAGAAGAAGA" meltingTemperature="54"><Component hybridizedRange="5286-5305" bases="CCCCGTAATGCAGAAGAAGA"/></BindingSite></Primer><Primer recentID="41" name="mCherry-R" sequence="TTGGTCACCTTCAGCTTGG" description="mCherry, reverse primer"><BindingSite location="5018-5036" boundStrand="1" annealedBases="TTGGTCACCTTCAGCTTGG" meltingTemperature="57"><Component hybridizedRange="5018-5036" bases="TTGGTCACCTTCAGCTTGG"/></BindingSite><BindingSite simplified="1" location="5018-5036" boundStrand="1" annealedBases="TTGGTCACCTTCAGCTTGG" meltingTemperature="57"><Component hybridizedRange="5018-5036" bases="TTGGTCACCTTCAGCTTGG"/></BindingSite></Primer><Primer recentID="44" name="mU6-F" sequence="CAGCACAAAAGGAAACTCACC" description="Mouse U6 promoter, forward primer"><BindingSite location="2765-2785" boundStrand="0" annealedBases="CAGCACAAAAGGAAACTCACC" meltingTemperature="56"><Component hybridizedRange="2765-2785" bases="CAGCACAAAAGGAAACTCACC"/></BindingSite><BindingSite simplified="1" location="2765-2785" boundStrand="0" annealedBases="CAGCACAAAAGGAAACTCACC" meltingTemperature="56"><Component hybridizedRange="2765-2785" bases="CAGCACAAAAGGAAACTCACC"/></BindingSite></Primer><Primer recentID="51" name="pBluescriptKS" sequence="TCGAGGTCGACGGTATC" description="For pBluescript vector"><BindingSite location="6284-6300" boundStrand="1" annealedBases="TCGAGGTCGACGGTATC" meltingTemperature="54"><Component hybridizedRange="6284-6300" bases="TCGAGGTCGACGGTATC"/></BindingSite><BindingSite simplified="1" location="6284-6300" boundStrand="1" annealedBases="TCGAGGTCGACGGTATC" meltingTemperature="54"><Component hybridizedRange="6284-6300" bases="TCGAGGTCGACGGTATC"/></BindingSite></Primer><Primer recentID="54" name="pBR322ori-F" sequence="GGGAAACGCCTGGTATCTTT" description="pBR322 origin, forward primer"><BindingSite location="7131-7150" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="7131-7150" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite><BindingSite simplified="1" location="7131-7150" boundStrand="1" annealedBases="GGGAAACGCCTGGTATCTTT" meltingTemperature="56"><Component hybridizedRange="7131-7150" bases="GGGAAACGCCTGGTATCTTT"/></BindingSite></Primer><Primer recentID="69" name="pRS-marker" sequence="CGGCATCAGAGCAGATTGTA" description="pRS vectors, use to sequence yeast selectable marker"><BindingSite location="8850-8869" boundStrand="1" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="8850-8869" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite><BindingSite simplified="1" location="8850-8869" boundStrand="1" annealedBases="CGGCATCAGAGCAGATTGTA" meltingTemperature="54"><Component hybridizedRange="8850-8869" bases="CGGCATCAGAGCAGATTGTA"/></BindingSite></Primer><Primer recentID="70" name="Puro-F" sequence="GCAACCTCCCCTTCTACGAGC" description="Puromycin resistance gene, forward primer"><BindingSite location="4697-4717" boundStrand="0" annealedBases="GCAACCTCCCCTTCTACGAGC" meltingTemperature="60"><Component hybridizedRange="4697-4717" bases="GCAACCTCCCCTTCTACGAGC"/></BindingSite><BindingSite simplified="1" location="4697-4717" boundStrand="0" annealedBases="GCAACCTCCCCTTCTACGAGC" meltingTemperature="60"><Component hybridizedRange="4697-4717" bases="GCAACCTCCCCTTCTACGAGC"/></BindingSite></Primer><Primer recentID="71" name="Puro-R" sequence="GTGGGCTTGTACTCGGTCAT" description="Puromycin resistance gene, reverse primer. Also called puro-variant-R"><BindingSite location="4201-4220" boundStrand="1" annealedBases="GTGGGCTTGTACTCGGTCAT" meltingTemperature="59"><Component hybridizedRange="4201-4220" bases="GTGGGCTTGTACTCGGTCAT"/></BindingSite><BindingSite location="4483-4492" boundStrand="1" annealedBases="ACTCGGCAT" meltingTemperature="23"><Component bases="GTGGGCTTGT"/><Component hybridizedRange="4487-4492" bases="ACTCGG"/><Component bases="T"/><Component hybridizedRange="4483-4485" bases="CAT"/></BindingSite><BindingSite simplified="1" location="4201-4220" boundStrand="1" annealedBases="GTGGGCTTGTACTCGGTCAT" meltingTemperature="59"><Component hybridizedRange="4201-4220" bases="GTGGGCTTGTACTCGGTCAT"/></BindingSite><BindingSite simplified="1" location="4483-4492" boundStrand="1" annealedBases="ACTCGGCAT" meltingTemperature="23"><Component bases="GTGGGCTTGT"/><Component hybridizedRange="4487-4492" bases="ACTCGG"/><Component bases="T"/><Component hybridizedRange="4483-4485" bases="CAT"/></BindingSite></Primer><Primer recentID="82" name="WPRE-R" sequence="CATAGCGTAAAAGGAGCAACA" description="WPRE, reverse primer"><BindingSite location="1814-1825" boundStrand="0" annealedBases="AAAGGACAACA" meltingTemperature="26"><Component bases="CATAGCGTA"/><Component hybridizedRange="1814-1819" bases="AAAGGA"/><Component bases="G"/><Component hybridizedRange="1821-1825" bases="CAACA"/></BindingSite><BindingSite location="5745-5765" boundStrand="1" annealedBases="CATAGCGTAAAAGGAGCAACA" meltingTemperature="55"><Component hybridizedRange="5745-5765" bases="CATAGCGTAAAAGGAGCAACA"/></BindingSite><BindingSite simplified="1" location="1814-1825" boundStrand="0" annealedBases="AAAGGACAACA" meltingTemperature="26"><Component bases="CATAGCGTA"/><Component hybridizedRange="1814-1819" bases="AAAGGA"/><Component bases="G"/><Component hybridizedRange="1821-1825" bases="CAACA"/></BindingSite><BindingSite simplified="1" location="5745-5765" boundStrand="1" annealedBases="CATAGCGTAAAAGGAGCAACA" meltingTemperature="55"><Component hybridizedRange="5745-5765" bases="CATAGCGTAAAAGGAGCAACA"/></BindingSite></Primer></Primers>

  .<?xml version="1.0"?><Features nextValidID="22"><Feature recentID="0" name="loxP" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5604-5637" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="loxP" directionality="2" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2729-2762" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;Cre recombinase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8116776/&quot;&gt;Shaw et al., 2021&lt;/a&gt;).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="KS primer" directionality="2" type="primer_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6284-6300" color="#a020f0" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;common sequencing primer, one of multiple similar variants&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="Factor Xa site" directionality="2" translationMW="473.53" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6163" readingFrame="-1" consecutiveTranslationNumbering="1" maxRunOn="6089" maxFusedRunOn="6089" detectionMode="exactProteinMatch"><Segment range="6164-6175" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Factor Xa recognition and cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="IEGR"/></Q></Feature><Feature recentID="4" name="gp41 peptide" directionality="1" translationMW="1816.04" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2364" maxFusedRunOn="2364" detectionMode="exactProteinMatch"><Segment range="2017-2061" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;recognized by the 2H10 single-chain llama nanobody&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/23505368&quot;&gt;Lutje Hulsik et al., 2013&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="KNEQELLELDKWASL"/></Q></Feature><Feature recentID="5" name="T2A" directionality="1" translationMW="1829.96" type="CDS" allowSegmentOverlaps="0" cleavageArrows="4858" consecutiveTranslationNumbering="1" consecutiveNumberingStartsFrom="139" maxRunOn="5584" maxFusedRunOn="5584" detectionMode="exactProteinMatch"><Segment range="4808-4861" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;2A peptide from&amp;nbsp;&lt;i&gt;Thosea asigna&lt;/i&gt; virus capsid protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="protein_id"><V text="&lt;html&gt;&lt;body&gt;&lt;br&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="EGRGSLLTCGDVEENPGP"/></Q></Feature><Feature recentID="6" name="cPPT/CTS" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2359-2476" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;central polypurine tract and central termination sequence of HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="RRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1599-1832" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="HIV-1 Ψ" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="977-1102" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;packaging signal of human immunodeficiency virus type 1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6810-6990" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="5' LTR (truncated)" type="LTR" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="750-930" color="#ffe4c4" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;truncated 5' long terminal repeat (LTR) from HIV-1&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="WPRE" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5693-6281" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;woodchuck hepatitis virus&amp;nbsp;posttranscriptional regulatory element&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="EF-1α core promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3031-3242" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;core&amp;nbsp;promoter for human elongation factor EF-1α&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="EF-1α intron A" type="intron" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3243-4181" color="noColor" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;intron upstream of the start codon of human EF-1α&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="534-732" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="153-532" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="16" name="mCherry" directionality="1" translationMW="26722.25" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="4874-5584" color="#ff0000" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;mammalian codon-optimized&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;monomeric derivative of DsRed fluorescent protein (&lt;a href=&quot;http://www.ncbi.nlm.nih.gov/pubmed/15558047&quot; title=&quot;http://www.ncbi.nlm.nih.gov/pubmed/15558047&quot;&gt;Shaner et al., 2004&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTGGMDELYK*"/></Q></Feature><Feature recentID="17" name="Kozak sequence" type="regulatory" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4868-4877" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;vertebrate consensus sequence for strong initiation of translation (&lt;a href=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot; title=&quot;https://www.ncbi.nlm.nih.gov/pubmed/3313277&quot;&gt;Kozak, 1987&lt;/a&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="regulatory_class"><V predef="other"/></Q></Feature><Feature recentID="18" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="7052-7640" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="19" name="PuroR" directionality="1" translationMW="21496.48" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="5584" maxFusedRunOn="5584"><Segment range="4202-4798" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;pac&lt;/i&gt; from &lt;i&gt;Streptomyces alboniger&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to puromycin&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;puromycin &lt;i&gt;N&lt;/i&gt;-acetyltransferase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"/></Q></Feature><Feature recentID="20" name="AmpR" directionality="2" translationMW="31557.36" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="8602" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="7811-8602" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="8603-8671" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;β-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature><Feature recentID="21" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="8672-8776" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   )<?xml version="1.0"?><CustomEnzymeSets/>
